Abstract
We present results on the analysis of 100 mL medium samples extracted from sterilized foam (Smithers-Oasis, Kent OH) used to support the growth of a representative dicotyledon ( Haplopappus gracilis) and a representative monocotyledon ( Hemerocallis cv Autumn Blaze) in NASA’s Plant Growth Unit (PGU) during a 5-day Space Shuttle flight and ground experiments. At recovery, the media remaining within replicate ( n = 5) foam blocks (for both the spaceflight and ground experiments) were extracted under vacuum, filtered and subjected to elemental analyses. A unique aspect of this experiment was that all plants were either aseptically-generated tissue culture propagated plantlets or aseptic seedling clones. The design of the PGU facilitated the maintenance of asepsis throughout the mission (confirmed by post-flight microbial sampling) and thus any possible impact of microorganisms on medium composition was eliminated. Concentration levels of some elements remained the same, while some decreased and others increased. There was a significant two-fold difference between the final concentrations of potassium when the Earth-based and microgravity experiments were contrasted.
Published Version
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