Changes in Physiology and Stress Responses of Pellet‐Reared Largemouth Bass Fed Live‐Forage Diets

Abstract

AbstractAdvanced‐sized Largemouth Bass Micropterus salmoides to be used for stocking and experimentation can be efficiently reared on prepared feed in intensive culture conditions, but formulated diets containing high levels (≥20%) of dietary carbohydrates may lead to high liver and muscle lipid and high liver glycogen levels, which may affect survival and stress responses. Largemouth Bass were raised to a size of 240–344 mm TL on formulated diets and then were fed live forage (i.e., naturalization); the effects of naturalization on liver, blood, and muscle health indices and physiological stress measures were evaluated, and indices were compared with those of wild Largemouth Bass from three different reservoirs. Hepatosomatic index (HSI) decreased by week 1 of naturalization; liver glycogen decreased by week 2; liver lightness, yellowness, and steatosis decreased by week 4; and liver moisture and lipid concentration stabilized after 4 weeks. Plasma cortisol was higher by week 1 of naturalization than by week 6 and onward, and plasma pH decreased after 1 week. Muscle color showed changes by week 1 of naturalization, muscle collagen stabilized by week 4, and muscle lipids decreased more gradually compared to the liver. The magnitude of stress response in fish subjected to a 60‐s chasing stressor was unaffected by naturalization. Among populations of wild fish, many liver, blood, and muscle metrics were similar, with the exception of liver yellowness, glycogen, and HSI; muscle redness, lipids, and moisture; and the viscerosomatic index. Therefore, after rearing on a high‐carbohydrate, high‐lipid diet (i.e., 20% carbohydrates; 16% lipids), 4–6 weeks of naturalization can improve liver health, with most liver parameters being similar to those found in populations of wild fish.