Changes in physiological traits and expression of key genes involved in sugar signaling pathway in rice under high temperature stress.

Abstract

Efficient assimilate partitioning between the source and sink organs to achieve increased grain weight is coordinated by the sugar signaling mechanism. The expression of the genes involved in sugar signaling mainly hexokinases 2 (OsHXK2), Sucrose-nonfermentation1-related protein kinase1 (OsSnRK1), trehalose-6-phosphate synthase 1 (OsTPS1) and target of rapamycin (OsTOR) under high temperature stress was examined in tolerant (NL-44) and susceptible (Vandana) varieties of rice. The photosynthetic rate, stomatal conductance, water-use efficiency, photochemical efficiency (Fv/Fm), quantum yield (ϕPSII), pollen viability, spikelet fertility and 1000 grain weight were significantly higher in NL-44 compared to Vandana under stress. The difference in the gene expression levels in the vegetative and grain-filling phases as well as between the tolerant and susceptible varieties, revealed unique pathways of sugar signaling under heat stress. In the vegetative phase, the expression of OsTOR seems to be the difference between NL-44 and Vandana for their differed heat stress tolerance whereas, in the grain-filling phase, the difference between the varieties lay in the regulation of OsHXK2. The comparative changes in the expression levels between the genes under the varying conditions indicate the sugar status in the source and sink organs that are available for translocation or remobilization.