Abstract
Chronic UV radiation causes oxidative stress and inflammation of skin and blood cells. Therefore, in this study, we assessed the effects of cannabidiol (CBD), a natural phytocannabinoid with antioxidant and anti-inflammatory properties, on the phospholipid (PL) and ceramide (CER) profiles in the plasma of nude rats irradiated with UVA/UVB and treated topically with CBD. The results obtained showed that UVA/UVB radiation increased the levels of phosphatidylcholines, lysophospholipids, and eicosanoids (PGE2, TxB2), while downregulation of sphingomyelins led to an increase in CER[NS] and CER[NDS]. Topical application of CBD to the skin of control rats significantly upregulated plasma ether-linked phosphatidylethanolamines (PEo) and ceramides. However, CBD administered to rats irradiated with UVA/UVB promoted further upregulation of CER and PEo and led to significant downregulation of lysophospholipids. This was accompanied by the anti-inflammatory effect of CBD, manifested by a reduction in the levels of proinflammatory PGE2 and TxB2 and a dramatic increase in the level of anti-inflammatory LPXA4. It can therefore be suggested that topical application of CBD to the skin of rats exposed to UVA/UVB radiation prevents changes in plasma phospholipid profile resulting in a reduction of inflammation by reducing the level of LPE and LPC species and increasing antioxidant capacity due to upregulation of PEo species.
Highlights
UV phototherapy is currently one of the most widely used treatments for skin diseases [1,2]
The concentration of cannabidiol in the blood plasma of the animals, determined using LC–MS/MS, was 40.7 ± 7.8 pmol/mL in the group of rats to which CBD was applied (CBD group); 44.5 ± 9.3 pmol/mL in the group of rats irradiated with UVA and treated with CBD (UVA+CBD group); and 25.8 ± 6.2 pmol/mL in the group of animals irradiated with UVB and treated with CBD (UVB+CBD group), whereas a statistically significant difference, namely a decrease in the level of this fitocannabinoid, was observed only in the plasma of animals irradiated with UVB and treated with CBD
UV radiation is one of the main environmental factors that cause damage to skin cells plasma of rats exposed to UVA/UVB light and significantly reduced in the plasma of rats treated with CBD and irradiated with UVA/UVB (Figure 6)
Summary
UV phototherapy is currently one of the most widely used treatments for skin diseases [1,2]. Long-term skin exposure to UV radiation has a therapeutic effect, it causes redox imbalance, leading to the induction of oxidative stress in cells [3]. In such a situation, the metabolism of the main cellular components, proteins, and phospholipids is significantly altered. Research results so far have shown that CBD contributes to the reduction of ROS levels and the normalization of antioxidant parameters. It affects the level of endocannabinoids and supports them in the activation of G-protein-coupled receptors, including cannabinoid receptors as well as TRPVs and PPARs, which are involved in the regulation of ROS and proinflammatory cytokines levels, including TNFα [17,18,19]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
