Changes in microbial community structure and functioning of a semiarid soil due to the use of anaerobic digestate derived composts and rosemary plants

Abstract

We studied the changes in structure and functioning of the microbial community in a degraded agricultural soil after the addition of two composts, obtained from cattle manure or pig slurry anaerobic digestate, and the use of rosemary plants for restoring soil quality.The composts were added at low or high doses to soil samples (30 or 60tha−1, respectively), which were kept in microcosms for 6months. Some soil microcosms were treated with inorganic fertiliser and other non-treated soils were used as microbiological controls. Rosemary plants, used both for their ability to grow in semi-arid regions and for the capacity of their root system to protect soil from erosion, were planted in half of the entire microcosm set up. At different times (0–180days) microbial abundance and dehydrogenase activity were measured in the various experimental treatments. Total and water-soluble soil organic carbon and nitrogen contents were assessed at 0 and 180days. With an increase in carbon and nitrogen soil content, a rise in microbial abundance was also observed in the presence of both composts. However, microbial activity was significantly influenced by the presence of rosemary, without considering the allochthonous carbon and nitrogen input. Microbial community structure and diversity were also assessed by Fluorescence In Situ Hybridization in the different treatments. The highest values for microbial community biodiversity were found in the co-presence of rosemary and at low concentrations of both composts.The overall results suggest that the use of composts together with plant species suited to Mediterranean areas seems to be an appropriate strategy for restoring soil quality and the ecosystem services provided by microorganisms.