Abstract
β-casein is a primary protein in milk, and its variants have been associated with changes in the protein content of bovine milk. However, there has been little research focused on the effects of β-casein variants on milk metabolites. In the present study, dairy cows producing milk with β-casein variant A1/A1 (A1), A2/A2 (A2), and their heterozygote A1/A2 (A12) were screened by a high-resolution melting method. Individual milk samples were then collected from each of the cows, and the milk metabolites were separated and analyzed using nuclear magnetic resonance spectroscopy- and liquid-chromatography mass spectrometry-based metabolomics techniques. Differences in metabolites among the variant groups were evaluated by multivariate statistical analysis. The relative abundances of methionine, proline, and α-lactose were the highest in β-casein variant A2 milk, whereas choline, glycine, citric acid, and cyclic adenosine monophosphate (cAMP) showed the highest abundances in variant A1 milk. Metabolic pathways analysis indicated that the differential metabolites between variants A1 and A2 were involved in pantothenate and coenzyme A biosynthesis, butanoate metabolism, and valine, leucine, and isoleucine biosynthesis. Our results reveal the differences in milk metabolites among the β-casein variants A1, A2, and the heterozygote. These findings, thus, provide novel insights into the effects of β-casein variants on milk metabolites, facilitating further research into the mechanism of the biosynthesis of milk components in the mammary gland and the potential physiological function of milk associated with β-casein variants.
Highlights
The specific changes in the metabolites of β-casein variant milk remain unclear
Score plots and coefficient loading plots of orthogonal PLS-DA (OPLS-DA) were used to identify the differential metabolites between variants A1 and A2 or heterozygote A12 milk (Figure S3)
The metabolite profiles between variants A2 and heterozygote A12 were not significantly different based on the OPLS-DA, in which samples from these two groups were not clearly separated and the Q2 value was less than
Summary
The specific changes in the metabolites of β-casein variant milk remain unclear. The metabolites with the highest abundance were methionine, proline, and α-lactose in variant A2/A2 milk, choline, glycine, citric acid, and cyclic adenosine monophosphate (cAMP) in variant A1/A1 milk, and uric acid and cytosine in heterozygote milk. These results may facilitate further explorations of the differences in the biosynthesis of milk components in the mammary gland and help to elucidate the potential influence of β-casein variants on the physiological function of milk. Milk with bovine β-casein variants A2 and A1 was reported to induce the formation of a yogurt gel microstructure [10] These findings indicate that differences in milk protein components could be related to β-casein variants. Little information is available on the changes in milk metabolites induced by bovine β-casein variants
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