Changes in metabolic activities of hepatoma cells following increasing x-ray doses.

Abstract

IT has been reported that aerobic and anaerobic glycolysis in the Yoshida ascites sarcoma are markedly impaired following in vitro irradiation with a dose of 20 kr. of X-rays. In these conditions uptake of oxygen is slightly decreased1. A lowered incorporation of alanine labelled with carbon-14 into protein of the ascites-sarcoma, cells was also noted under anaerobic conditions2. More recently the in vitro irradiation of Ehrlich ascites tumour cells with different doses of X-rays (20–300 kr.), was found to stimulate both oxygen consumption and aerobic glycolysis whereas anaerobic glycolysis decreased as the X-ray dose increased up to about 200 kr. (ref. 3).