Abstract
Lipids were extracted with chloroform-methanol from Ceiba pentandra seeds at different stages of maturity, separated by TLC and tested for the Halphen response. Cyclopropene fatty acids (CFA) were absent from mono- and diglycerides and phospholipids. Free fatty acids, diglycerides and phospholipids were maximum on the 23rd, 65th and 65th day respectively and minimum on the 96th day (mature) after flowering. The fatty acid compositions were determined by GLC. Dihydromalvalic acid reached a maximum value on the 81st day, while malvalic acid remained almost constant from the 38th day and sterculic acid steadily increased. Dihydrosterculic acid was noticed only in the free fatty acids and phospholipids. The positional distribution in the triglycerides was determined by lipolysis. Since CFA inhibited lipolysis partially the triglycerides were dissolved in chloroform and treated with methanolic silver nitrate prior to lipolysis. CFA were found in the 2-position, though to a lesser extent than in the primary positions, indicating rearrangement of glycerides during maturation.
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