Abstract
Three monoclonal antibodies that stained acrosin of 55, 53, 45 and 38 kDa (ACR.2), acrosomal protein of 28 kDa (ACR.4) and sperm protein of 17 kDa (ACR.3) were used for immunostaining and immunoblotting analysis of boar spermatozoa before and during capacitation and ionophore-induced acrosome reaction. Two proteins (acrosin and the 28 kDa protein) showed similar changes in the acrosome during capacitation and induced acrosome reaction. For detection of these proteins, immunoelectron microscopy was also used. The immunofluorescence test revealed that most spermatozoa lost acrosin and the 28 kDa protein after incubation with ionophore A 23187. The lost proteins were detected in the culture medium by immunoblotting. During capacitation and induced acrosome reaction, sperm protein of 17 kDa (ACR.3) showed a different pattern of labelling. The changes in relocation of specific proteins during capacitation and acrosome reaction support a concept of physiological preparation of spermatozoa for sperm-egg interaction.
Published Version
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