Abstract
Two substrains ofLeishmania donovani strain 3S were used in a study of grwoth rates of promastigote and amastigote stages as well as of infectivity of the latter stages in the course of cultivation, animal passages, and heat adaptation. One of these substrains, 3S-25A, was initiated with amastigotes of strain 3S maintained by serial passages in golden hamsters since 1962. The 3S-25A promastigotes were transferred 24 times (about 142 generations), then passaged once in a hamster. The amastigotes derived from this hamster on postinoculation day 30 were employed for initiation of culture promastigotes designated as substrain 3S-25A′. This culture was transferred 20 times (about 141 generations). The third substrain, 3S-37, was isolated in culture in 1974, then adapted to 37° C. All cultures were grown in modified Tobie's medium (Tm). Young C57BL/6J mice were employed in the estimation of generation times (G) of amastigote stages and infectivity of promastigotes.
Published Version
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