Changes in gill morphology of Atlantic salmon ( Salmo salar) smolts due to addition of acid and aluminum to stream water

Abstract

One-year-old Atlantic salmon smolts were held in three artificial channels adjacent to a softwater (mean sp. cond. 30 μS cm −1, circumneutral stream. Water in one channel was untreated (mean pH 6.25); the others received additions of acid (to mean pH 5.6), or acid plus aluminum (to mean pH 5.5; mean exchangeable Al 158 μg litre −1). Gills were sampled after 16 and 23 days of exposure for morphometric examination. On primary lamellae, chloride cells were more numerous in both experimental treatments than in controls. In contrast, numbers of chloride cells on secondary lamellae were elevated only in fish exposed to acid without added Al. Chloride cell size and shape also varied with time and treatment. Fewer gill mucous cells were found in fish exposed to acid plus Al than in controls. Chloride cell proliferation and structural changes may represent an attempt to compensate for increased ionic effluxes with low pH stress by increasing uptake. However, if Al concentrations are high, chloride cells do not proliferate along the secondary lamellae, or proliferating cells are damaged and lost. This may limit the potential to increase ionic uptake.