Abstract
The study is aimed at investigating the changes in expressions of heat shock protein 27 (HSP27), HSP70, and soluble glycoprotein (SGP) in heart failure (HF) rats complicated with pulmonary edema and exploring their potential correlations with cardiopulmonary functions. The rat model of HF was established, and the rats were divided into HF model group (model group, n = 15) and normal group (n = 15). After successful modeling, MRI and ECG were applied to detect the cardiac function indexes of the rats. The myocardial function indexes were determined, the injury of myocardial tissues was observed via hematoxylin and eosin (HE) staining, and the content of myeloperoxidase (MPO), matrix metalloproteinase-9 (MMP-9), and tumor necrosis factor-alpha (TNF-α) in the blood was measured. The partial pressure of oxygen (PaO2) and oxygenation index (OI) were observed, and the airway resistance and lung compliance were examined. Moreover, quantitative polymerase chain reaction (qPCR) and Western blotting assay were performed to detect the gene and protein expression levels of HSP27, HSP70, and SGP130. The levels of serum creatine kinase (CK), creatine (Cr), and blood urea nitrogen (BUN) were increased markedly in model group (p < 0.05). Model group had notably decreased fractional shortening (FS) and ejection fraction (EF) compared with normal group (p < 0.05), while the opposite results of left ventricular end-diastolic diameter (LVEDD) and left ventricular end-systolic diameter (LVESD) were detected. In model group, the content of serum MPO, MMP-9, and TNF-α was raised remarkably (p < 0.05), OI and PaO2 were reduced notably (p < 0.05), the airway resistance was increased (p < 0.05), and the lung compliance was decreased (p < 0.05). Obviously elevated gene and protein expression levels of HSP27, HSP70, and SGP130 were detected in model group (p < 0.05). The expressions of HSP27, HSP70, and SGP130 are increased in HF rats complicated with pulmonary edema, seriously affecting the cardiopulmonary functions of the rats.
Highlights
Heart failure (HF) seriously threatens the human health, whose incidence rate, according to estimates, is rising persistently around the world, especially in the elderly people [1, 2]
Understanding the mechanism of HF complicated with pulmonary edema can relieve the symptoms rapidly and ameliorate the prognosis
This research aims to investigate the changes in expressions of heat shock protein 27 (HSP27), HSP70, and soluble glycoprotein (SGP) in HF rats complicated with pulmonary edema and their potential correlations with the cardiopulmonary functions
Summary
Heart failure (HF) seriously threatens the human health, whose incidence rate, according to estimates, is rising persistently around the world, especially in the elderly people [1, 2]. Pulmonary edema is the most common complication and a leading cause of HF. HF complicated with pulmonary edema is associated with the heart, kidney, and liver injuries in addition to the harmful effects on quality of life, thereby aggravating the clinical outcomes [4, 5]. With manifestations as BioMed Research International heart, kidney, lung and liquid imbalance, HF is recognized as an inflammatory disease since proinflammatory substances in high concentrations are detected in multiple vital organs such as heart, kidney, and lung and circulation systems [7]. The exact potential cause of inflammation has not been clarified yet, it is probably triggered by internal injuries
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
