Abstract
Changes in expression of P2X receptors (P2X 1–7) during postnatal development of the rat cerebellum are described. At P3, immunoreactivity (ir) to all the P2X receptors, except for P2X 3 receptors, was found in Purkinje cells and deep cerebellar nuclei, P2X 5-ir being most prominent. Granular and microglial cells were labeled for P2X 5 (weakly) and P2X 4 receptors, respectively. At P7, expression of all the P2X receptors (with the exception of P2X 3) was up-regulated, P2X 5 and P2X 6 receptors being most prominent. Scattered P2X receptor-ir in unipolar brush cells in the granular cell layer and P2X 1- and P2X 7-ir of microglial cells was also present. At P14, the dendritic trees of Purkinje cells were intensely labeled by P2X 1–7 receptor antibodies, except for P2X 3, while P2X 1, P2X 4 and P2X 7 receptor immunostaining in microglial cells and P2X 5 receptor immunostaining in granular cells was up-regulated. At P21, expression of all P2X receptors (except P2X 3) was down-regulated in the Purkinje cells and deep cerebellar nuclei; P2X 1, P2X 4 and P2X 7 receptors-ir was present in microglial cells. In contrast, expression of P2X 5-ir in granular cells was up-regulated. At P60, expression levels of all the P2X receptors (except P2X 3) were similar with those at P21. In double-labeling experiments, almost all the P2X-ir Purkinje cells were immunoreactive for calbindin-D28k, while 60–80% of P2X-ir cells in the granular cell layer were immunoreactive for calretinin. The possible short- and long-term functional significance of the changes in expression of P2X receptors during postnatal development is discussed.
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