Change of C/A at the SNP -592 of human IL-10 gene increase serum IL-10 levels in patients with cervical lesions and cervical cancer

Abstract

Event Abstract Back to Event Change of C/A at the SNP −592 of human IL-10 gene increase serum IL-10 levels in patients with cervical lesions and cervical cancer KIRVIS TORRES-POVEDA1, MARGARITA BAHENA-ROMÁN1, ALFREDO LAGUNAS-MARTÍNEZ1, KARINA DELGADO-ROMERO2, DAVID CANTÚ3, ALEJANDRO GARCÍA-CARRANCÁ3 and VICENTE MADRID-MARINA1* 1 INSTITUTO NACIONAL DE SALUD PUBLICA MEXICO, CHRONIC INFECTIONS AND CANCER DIVISION, Mexico 2 HEALTH SERVICES OF STATE OF MORELOS, CAPASAM, Mexico 3 INSTITUTO NACIONAL DE CANCEROLOGÍA MEXICO, Mexico An immunosuppressive state had been identified in women with human papillomavirus (HPV) persistent infection, characterized by high levels of interleukin (IL)-10 at cervix level. We have demonstrated that the -592 SNP in IL-10 gene promoter is associated with increased risk of cervical lesions (CL) and cervical cancer (CC). We analyzed whether exist significative differences of serum IL-10 protein between CL and CC cases and HPV negatives controls. Additionally, whether exchange in this SNP results in increased IL-10 gene promoter activity in HPV transformed cells. Using a cross-sectional design, peripheral blood samples of CL patients (n=204), with CC (n=80) and without CL HPV positives (n=66) and HPV negatives (n=100) were used to evaluate seric concentrations of IL-10 by ELISA. Transient transfection assay was performed in C33 and HeLa cells with two luciferase constructs containing the IL-10 promoter (C allele or A allele) (Figure 1). The serum IL-10 protein, were significantly higher in CL and CC cases (p<0.01), being higher in patients carrying the risk allele A (Figure 2). The mean levels of IL-10 increased in consecutive clinical stages of the CC. The C to A nucleotide exchange results in increased IL-10 gene promoter activity in C33 an HPV transformed cell line. In conclusion, we demonstrated that the C to A nucleotide exchange in this SNP results in increased IL-10 gene promoter activity in HPV transformed cells, supporting its role as an activator element and that be associated with the expression regulation of IL-10 at systemic level, which was progressively higher in CL and CC cases. Figure 1 Figure 2 References Torres-Poveda K, Burguete-García AI, Cruz M, Martínez-Nava GA, Bahena-Román M, Ortíz-Flores E, Ramírez-González A, López-Estrada G, Delgado-Romero K and Madrid-Marina V. The SNP at -592 of Human IL-10 Gene is associated with serum IL-10 levels and Increased Risk for Human Papillomavirus Cervical Lesion Development. Infectious Agents and Cancer 2012; 7(1):32-40. Keywords: Interleukin-10, SNP, Cervical lesion, Cervical Intraepithelial Neoplasia, Serum levels IL-10 Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune receptors and signaling Citation: TORRES-POVEDA K, BAHENA-ROMÁN M, LAGUNAS-MARTÍNEZ A, DELGADO-ROMERO K, CANTÚ D, GARCÍA-CARRANCÁ A and MADRID-MARINA V (2013). Change of C/A at the SNP −592 of human IL-10 gene increase serum IL-10 levels in patients with cervical lesions and cervical cancer. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00430 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 10 Apr 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. VICENTE MADRID-MARINA, INSTITUTO NACIONAL DE SALUD PUBLICA MEXICO, CHRONIC INFECTIONS AND CANCER DIVISION, Cuernavaca, MORELOS, 62100, Mexico, vmarina@insp.mx Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers KIRVIS TORRES-POVEDA MARGARITA BAHENA-ROMÁN ALFREDO LAGUNAS-MARTÍNEZ KARINA DELGADO-ROMERO DAVID CANTÚ ALEJANDRO GARCÍA-CARRANCÁ VICENTE MADRID-MARINA Google KIRVIS TORRES-POVEDA MARGARITA BAHENA-ROMÁN ALFREDO LAGUNAS-MARTÍNEZ KARINA DELGADO-ROMERO DAVID CANTÚ ALEJANDRO GARCÍA-CARRANCÁ VICENTE MADRID-MARINA Google Scholar KIRVIS TORRES-POVEDA MARGARITA BAHENA-ROMÁN ALFREDO LAGUNAS-MARTÍNEZ KARINA DELGADO-ROMERO DAVID CANTÚ ALEJANDRO GARCÍA-CARRANCÁ VICENTE MADRID-MARINA PubMed KIRVIS TORRES-POVEDA MARGARITA BAHENA-ROMÁN ALFREDO LAGUNAS-MARTÍNEZ KARINA DELGADO-ROMERO DAVID CANTÚ ALEJANDRO GARCÍA-CARRANCÁ VICENTE MADRID-MARINA Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.