Abstract
Lake whitefish ( Coregonus clupeaformis (Mitchill, 1818)) is an important commercial and recreational species in the Great Lakes. Precise age estimates are important for management, and two widely used techniques for otolith preparation are thin-section and crack-and-burn, which have not been compared for lake whitefish. Sagittal otoliths were collected from 92 lake whitefish in Green Bay and Lake Michigan and aged using thin-section and crack-and-burn techniques. Otoliths were aged independently by three individuals (two novices and one expert) to assess repeatability in estimated ages. Our investigation highlights the inherent difficulty of aging lake whitefish, where thin-section produced significantly older estimated ages (6–30 years) compared to crack-and-burn (5–26 years). Percent agreement of estimated ages between preparation techniques was low for all readers within ±0 years but increased when tolerance buffers were applied. Mean coefficient of variation values from both experience levels (>10.8%) exceeded the acceptable range reported in the literature (5%–7%); however, species longevity and nature of the structure must be considered when establishing target values. Variation in estimated ages is attributed to the experience level and interpretation of structural features. Species-specific training and establishing an objective framework to identify annuli will improve precision metrics.
Published Version
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