Abstract
Objectives:. The objectives of present study are: 1) To compare sterilizing effect on gutta-percha points of five disinfectant solutions: Povidone iodine (5%), Sodium hypochlorite (5%), Ethyl alcohol (95%), Hydrogen peroxide (3%) and a combination of Chlorhexidine (1.5%) and Cetrimide (15%) in equal proportions. 2) To find out which disinfectant solution requires less time for effective sterilization of gutta-percha points. Study design: Gutta-percha cones exposed to dental chair-side clinical environment were treated with above mentioned disinfectant solutions for 1, 3, 5, 7 and 10 minutes. They were later cultured into thioglycolate media at 37° C and observed after 24, 48 and 72 hours for turbidity. Those showing turbidity were sub-cultured for aerobic bacteria in blood agar, MacConkey's agar and chocolate agar at 37° C for 24-48 hours and for anaerobic bacteria in anaerobic blood agar at 37° C for 5 days. Microorganisms were identified by Gram stain and standard microbiological techniques. Results: The results showed that Sodium hypochlorite (5%) and a combination of Chlorhexidine (1.5%) and Cetrimide (15%) were found to be the most effective chemical disinfectants in sterilizing gutta-percha. Conclusion: All the disinfectant agents used in the study were found to be effective in sterilizing the gutta-percha points. However, there were differences in the time taken by these solutions in disinfecting the gutta-percha points. Sodium hypochlorite (5%) and combination of Chlorhexidine (1.5%) + Cetrimide (15%) achieved disinfection of the gutta-percha cones with the immersion time of 1 minute.
Published Version
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