Abstract
BackgroundCytokines and cytokine receptor concentrations increase in plasma and cerebrospinal fluid (CSF) of patients following subarachnoid haemorrhage (SAH). The relationship between plasma and CSF cytokines, and factors affecting this, are not clear.MethodsTo help define the relationship, paired plasma and cerebrospinal fluid (CSF) samples were collected from patients subject to ventriculostomy. Concentrations of key inflammatory cytokines, interleukin (IL)-1ß, IL-1 receptor antagonist (IL-1Ra), IL-1 receptor 2, IL-6, IL-8, IL-10, tumour necrosis factor (TNF)-α, and TNF receptors (TNF-R) 1 and 2 were determined by immunoassay of CSF and plasma from 21 patients, where samples were available at three or more time points.ResultsPlasma concentrations of IL-1ß, IL-1Ra, IL-10, TNF-α and TNF-R1 were similar to those in CSF. Plasma TNF-R2 and IL-1R2 concentrations were higher than in CSF. Concentrations of IL-8 and IL-6 in CSF were approximately10 to 1,000-fold higher than in plasma. There was a weak correlation between CSF and plasma IL-8 concentrations (r = 0.26), but no correlation for IL-6. Differences between the central and peripheral pattern of IL-6 were associated with episodes of ventriculostomy-related infection (VRI). A VRI was associated with CSF IL-6 >10,000 pg/mL (P = 0.0002), although peripheral infection was not significantly associated with plasma IL-6.ConclusionsThese data suggest that plasma cytokine concentrations cannot be used to identify relative changes in the CSF, but that measurement of CSF IL-6 could provide a useful marker of VRI.
Highlights
Cytokines and cytokine receptor concentrations increase in plasma and cerebrospinal fluid (CSF) of patients following subarachnoid haemorrhage (SAH)
The primary objective here was to define the relationship between central and peripheral markers of inflammation, following SAH. To achieve this we undertook a prospective study to compare peripheral and central (CSF) inflammatory markers in patients with SAH. This was conducted as part of a larger study of delayed cerebral ischaemia in patients with SAH [10] and took advantage of the availability of CSF from patients who had external ventricular drain (EVD) placed as part of their clinical care
We have shown that the concentrations of many of the commonly measured markers were similar in plasma and CSF compartments and in some instances plasma markers were higher
Summary
Cytokines and cytokine receptor concentrations increase in plasma and cerebrospinal fluid (CSF) of patients following subarachnoid haemorrhage (SAH). The primary objective here was to define the relationship between central and peripheral markers of inflammation, following SAH. To achieve this we undertook a prospective study to compare peripheral (plasma) and central (CSF) inflammatory markers in patients with SAH. This was conducted as part of a larger study of delayed cerebral ischaemia in patients with SAH [10] and took advantage of the availability of CSF from patients who had EVDs placed as part of their clinical care. Since infection is a potent inflammatory stimulus, a secondary, retrospective analysis was undertaken to examine the extent to which peripheral infection or ventriculostomy-related infection (VRI) was associated with observed changes in peripheral or central IL-6
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
