Ceramide-Graphene Oxide Nanoparticles Enhance the Cytotoxicity and Reduce the Occurrence and Development of Breast Cancer Xenografts

Abstract

Ceramide exerts crucial effect on inducing tumor cell apoptosis, while its insolublility limits the application in treating tumors. In this study, we used NGO-PEG-PEI (NPP) and C6-NPP/Cer (NPP/C) as method to explore NPP/C’s effect and its anti-tumor ability on breast cancer. Confocal microscopy was used to detect the transfection efficiency in tumor cells. Breast cancer cells were treated with C6-NGO-PEG-PEI solution (control group) or NPP/C followed by analysis of cell proliferation and apoptosis by flow cytometry. C6-ceramide solution (control group) or NPP/C was administrated into nude mice with tumor followed by measuring tumor volume and size as well as cell proliferation and apoptosis. NGO-PEG-PEI could significantly enhance cell intake and inhibit cell proliferation and promote apoptosis. In vivo transplantation tumor model experiments showed that NPP/C could decrease tumor growth, slow down the multiplication rate and accelerate apoptosis. In conclusion, Ceramide-graphene oxide can inhibit tumor growth by inhibiting tumor cell growth and promoting cell apoptosis.