Abstract
Centriolar satellites are PCM-1-positive granules surrounding centrosomes. Proposed functions of the centriolar satellites include protein targeting to the centrosome, as well as communication between the centrosome and surrounding cytoplasm. CEP90 is a centriolar satellite protein that is critical for spindle pole integrity in mitotic cells. In this study, we examined the biological functions of CEP90 in interphase cells. CEP90 physically interacts with PCM-1 at centriolar satellites, and this interaction is essential for centrosomal accumulation of the centriolar satellites and eventually for primary cilia formation. CEP90 is also required for BBS4 loading on centriolar satellites and its localization in primary cilia. Our results imply that the assembly and transport of centriolar satellites are critical steps for primary cilia formation and ciliary protein recruitment.
Highlights
Centriolar satellites are electron-dense materials concentrated near the centrosome in various cell types [1,2]
Our results revealed that CEP90 is required for the centrosomal accumulation of centriolar satellites and eventually for primary cilia formation
The total cellular levels of PCM-1 were not affected by CEP90 depletion (Figure 1A), indicating that CEP90 is required for the centrosomal accumulation of PCM-1
Summary
Centriolar satellites are electron-dense materials concentrated near the centrosome in various cell types [1,2]. PCM-1 is considered a central scaffold of the centriolar satellites because knockdown of PCM-1 results in the disappearance of the electron-dense satellite structures [4]. PCM-1 depletion makes other satellite proteins lose their typical localization patterns near centrosomes [5,6,7,8]. Another group of proteins is responsible for the subcellular distribution of centriolar satellites rather than existence. Additional members should be discovered to understand subcellular behaviors of centriolar satellites
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