Abstract
The use of a centrifugal partition chromatographic reactor is investigated for the production of chiral amino acids from racemic mixtures. Chirally selective enzymatic hydrolysis of N-acetyl- l-methionine into acetic acid and l-methionine was carried out in the chromatographic reactor to demonstrate the concept of integrated reaction and separation in centrifugal partition chromatography (CPC). The products l-methionine and acetic acid, as well as the unconverted substrate, N-acetyl- d-methionine are obtained separately. An aqueous two-phase system, consisting of PEG 600, potassium phosphate and water was successfully applied as liquid–liquid two-phase system in CPC. A model is presented, which describes the reaction chromatograms on the basis of the independently measured partition and mass transfer coefficients of the individual (reacting) components. The model appears to be a valuable tool for optimizing the reaction–separation process.
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