Abstract
Oxidative stress is one of the major mechanisms that underlies the damage of pancreatic ?-cells and defects in insulin secretion in diabetes. As herbal preparations can alleviate oxidative stress through their redox-active secondary metabolites, in this study we investigated the cytoprotective effects of Centaurium erythraea extract (CEe) against H2O2- and SNP-induced oxidative/nitrosative stress in Rin-5F ?-cells. The antioxidant activity of CEe and its effect on cell survival and insulin expression/secretion were evaluated. The CEe increased cell viability and ameliorated the disturbance of redox homeostasis in H2O2- and SNP-treated cells by decreasing DNA damage, lipid peroxidation and protein S-glutathionylation. The CEe restored GSH homeostasis in H2O2-treated ?-cells and attenuated the SNP-induced disturbance of the GSH/ GSSG ratio. The H2O2- and SNP-induced disruption of CAT, GPx, GR, MnSOD and CuZnSOD activities was adjusted by the CEe towards control values, as well as mRNA and protein levels of GPx, MnSOD and CAT. The CEe increased insulin expression/secretion particularly in H2O2-treated ?-cells, which was in accordance with the more pronounced antioxidant effect of the CEe observed in H2O2-treated ?-cells as compared to SNP-treated cells. These findings support the beneficial effect of the CEe in preventing or slowing down ?-cell damage and dysfunction caused by oxidative/nitrosative stress during diabetes development.
Highlights
Diabetes is a complex metabolic disorder with insulin producing pancreatic β-cell loss and dysfunction at its core
A slight increase (5%) was observed when 0.05 mg/mL of the extract was applied, while concentrations of the Centaurium erythraea extract (CEe) of 0.1 and 0.25 mg/mL increased the number of viable cells by 15% and 26%, respectively
The methanol extract prepared from Centaurium erythraea (CEe) attenuates the H2O2- and sodium nitroprusside (SNP)-induced redox imbalance in pancreatic β-cells, thereby contributing to increased cell-viability and insulin expression and secretion
Summary
Diabetes is a complex metabolic disorder with insulin producing pancreatic β-cell loss and dysfunction at its core. Studies indicate that oxidative stress-activated redox-signaling pathways damage β-cells, cause defects in insulin expression and secretion, leading to eventual cell death [1]. In type 1 diabetes, β-cell death is driven by pro-inflammatory cytokines and free radical species such as the superoxide anion (O2-) and nitric oxide radical (NO) released from immune cells infiltrating pancreatic islets [2]. How to cite this article: Đorđević M, Grdović N, Mihailović M, Arambašić Jovanović 117. J, Uskoković A, Rajić J, Đorđević M, Tolić A, Mišić D, Šiler B, Poznanović G, Vidaković M, Dinić S. Centaurium erythraea extract reduces redox imbalance and improves insulin expression and secretion in pancreatic β-cells exposed to oxidative and nitrosative stress.
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