Abstract
Common effluent treatment plant (CETP) for tannery effluent, is the combination of physical, chemical and biological treatment to facilitate the degradation of industrial waste water. Obviously, the biomass which survives in this extreme environment may have the ability to utilize the effluent as the sole carbon source for its survival. The ultimate aim of the present investigation is to expose the microbial diversity in each stage of the CETP through the culture dependent way. Bacterial diversity in the effluent were analysed through 16S rRNA gene. The community study revealed the dominance of firmicutes and the dominant genus was Bacillus sp, with variable species diversity. Notably, Putative Bacillus sp, B. firmus and B. licheniformis were observed in all stages of treatment. The dominant residents were analysed by BProm and TF site scan to prove their uniqueness. This species richness indicates the capability of liveliness in treatment plant and whose can be exploited for treating the effluent by using modern molecular approach.AbbreviationsCETP - Common Effluent Treatment Plant, PTIET - Pallavaram Tanners Industrial Effluent Treatment Company Ltd.
Highlights
In the world’s foot wear production India occupies 10th position and is one of the highest producers of raw hides
The 16S product was resolved in 1.5% (w/v) agarose gel electrophoresis in 1X TAE buffer using 1 kb ladder as Result and Discussion: The present study was performed to estimate the microbial population in the different stages of treatment in Common effluent treatment plant (CETP)
A coherent dominance of putative Bacillus sp, B. firmus and B. licheniformis were observed in all the stages of effluent, while uncultured Bacillus sp was observed in all the treated samples except raw effluent and secondary treated
Summary
In the world’s foot wear production India occupies 10th position and is one of the highest producers of raw hides. The effluent samples were collected in sterilized containers from the raw effluent tank, primary treated, secondary treated, tertiary treated and soil from CETP, Pallavaram, Chennai, India. The 16S product was resolved in 1.5% (w/v) agarose gel electrophoresis in 1X TAE buffer using 1 kb ladder as Result and Discussion: The present study was performed to estimate the microbial population in the different stages of treatment in CETP.
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