Abstract
Techniques involving three-dimensional (3D) tissue structure reconstruction and analysis provide a better understanding of changes in molecules and function. We have developed AutoCUTS-LM, an automated system that allows the latest advances in 3D tissue reconstruction and cellular analysis developments using light microscopy on various tissues, including archived tissue. The workflow in this paper involved advanced tissue sampling methods of the human cerebral cortex, an automated serial section collection system, digital tissue library, cell detection using convolution neural network, 3D cell reconstruction, and advanced analysis. Our results demonstrated the detailed structure of pyramidal cells (number, volume, diameter, sphericity and orientation) and their 3D spatial organization are arranged in a columnar structure. The pipeline of these combined techniques provides a detailed analysis of tissues and cells in biology and pathology.
Highlights
Techniques involving three-dimensional (3D) tissue structure reconstruction and analysis provide a better understanding of changes in molecules and function
This is accomplished by modifying and adjusting the original AutoCUTS, which was designed for scanning electron microscopy (EM) array tomography[18,19,20], to image archival human brain tissues (~20 years) in layer III of Brodmann Area 46 (BA46)
A block of tissue from the dorsolateral prefrontal cortex that contained all of BA46 was removed from each brain[33], see Fig. 1
Summary
Techniques involving three-dimensional (3D) tissue structure reconstruction and analysis provide a better understanding of changes in molecules and function. Our results demonstrated the detailed structure of pyramidal cells (number, volume, diameter, sphericity and orientation) and their 3D spatial organization are arranged in a columnar structure The pipeline of these combined techniques provides a detailed analysis of tissues and cells in biology and pathology. We developed Automatic Collector of Ultrathin Sections for Light microscopy (AutoCUTSLM) to measure the neuronal cell morphology and their spatial organization in 3D-space of archived tissue. This is accomplished by modifying and adjusting the original AutoCUTS, which was designed for scanning EM array tomography[18,19,20], to image archival human brain tissues (~20 years) in layer III of Brodmann Area 46 (BA46). The 3D spatial arrangement and structural parameters of pyramidal neurons in layer III of BA46 were analyzed in three human brains applying recently developed methods
Sign-in/Register to view highlights & summary 
Published Version (
Free)
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call