Abstract
Cell wall material was isolated as water unextractable solids (WUS) from soybean meal. The isolation of WUS yields a fraction that contains 92% of the polysaccharides present in soybean meal and only a few other components. Arabinose, galactose, uronic acids and glucose (cellulose) were the major constituent sugars. WUS was sequentially extracted with chelating agent (chelating agent soluble solids, ChSS), dilute alkali (dilute alkali soluble solids, DASS), 1 m alkali (1 m alkali soluble solids, 1 m ASS) and 4 m alkali (4 m alkali soluble solids, 4 m ASS) to leave a cellulose-rich residue (RES). ChSS was the major extract, yielding 38% of the polysaccharides present in the WUS. All extracts and the residue were characterised by their sugar composition and their molecular-weight distribution. The extracts ChSS and DASS were fractionated by anion exchange chromatography. They showed identical elution patterns: an unbound fraction, five bound fractions of which one fraction eluted only with alkali. Anion exchange chromatography was also performed after saponification of both pectin-rich extracts, again resulting in identical elution patterns.
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