Abstract
Successful regeneration of tissues and organs relies on the application of suitable substrates or scaffolds in scaffold-based regenerative medicine. In this study, Ti-6Al-4V alloy films (Ti alloy film) were produced using a three-dimensional printing technique called Selective Laser Melting (SLM), which is one of the metal additive manufacturing techniques. The thickness of produced Ti alloy film was approximately 250 μm. The laser-irradiated surface of Ti alloy film had a relatively smooth yet porous surface. The non-irradiated surface was also porous but also retained a lot of partially melted Ti-6Al-4V powder. Cell proliferation ability of mouse fibroblast-like cells (L929 cells) and mouse osteoblast-like cells (MC3T3-E1 cells) on both the surfaces of Ti alloy film was examined using WST assay. Both L929 and MC3T3-E1 cells underwent cell proliferation during the culture period. These results indicate that selective laser melting is suitable for producing a cell-compatible Ti-6Al-4V alloy film for biomaterials applications.
Highlights
General clinical approaches in dentistry and medicine to the problems of injury, disease, or non-functional tissue/organ include reconstructive surgery and transplantation of artificial materials [1]
Selective Laser Melting (SLM) is a three-dimensional printing technique, and this technique was used to produce Ti alloy film in this study. This additive manufacturing (AM) technique allows the generation of complex three-dimensional metal parts by selectively melting successive layers of metal powder on top of each other, using the thermal energy supplied by a focused and computer-controlled laser beam [11] [12]
The laser-irradiated surface was relatively smooth with large numbers of pores these did not penetrate to the opposite side
Summary
General clinical approaches in dentistry and medicine to the problems of injury, disease, or non-functional tissue/organ include reconstructive surgery and transplantation of artificial materials [1]. Scaffolds for TE must have good biocompatibility, a highly porous and interconnected structure to allow cell migration, and mechanical properties that match those of the host tissue/organ [2] [3]. Titanium (Ti), for example, has excellent biocompatibility and corrosion resistance so has been used extensively in dental implants and orthopedic prostheses, such as hips and knees [9] [10]. To improve fixation and stability of dental and orthopedic implants, porous coatings are being developed that promote mechanical inter locking by osteoblast proliferation and bone ingrowth. It is assumed that this Ti-6Al-4V film will be clinically applied for regeneration of bone defects as a scaffold in the near future, cell proliferation ability and calcification ability of mouse osteoblast-like cells were examined
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