Abstract
The dynamics of cell renewal in the slowly proliferating epithelium of the mouse forestomach were analysed by the Method of Labeled Mitoses (pulse labeling with tritiated thymidine, serial killing, preparation of autoradiographs, determinations of fraction mitoses labeled between injection and sacrifice). The following values were estimated: T M about 1–2 hr; T G2 about 1–2 hr; T S about 13.5 hr. The estimate of T S is higher than that reported for more rapidly proliferating tissues and indicates that T S is not a true constant in all mouse tissues. Nevertheless, it was suggested that differences in cell cycle times may reflect primarily, but not exclusively, differences in T G1 and T G0 . Proliferative cells of the forestomach may undergo one of two possible proliferative cycles. Post-mitotic cells may either pass directly into a G 1-phase ( T c of about 30 hr) or be shunted into a moderately long G 0 -phase ( T C of about 2 to 4 weeks). The significance of this finding to the concept of cycle time is briefly discussed. Examples of G 0 cells in a few proliferating systems are cited.
Published Version
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