Abstract
Chemical immobilization of Trichosporon cutaneum R57 cells onto modified polyacrylonitrile granules and fibres was carried out. The polymer carriers were modified in water solutions of NaOH and 1,2-diaminoethane. The concentration of amino groups obtained by the modification was determined. The optimal conditions for chemical immobilization of cells of T. cutaneum R57 with glutaraldehyde were found to be: 40% NaOH concentration, 46.8 mg/ml initial cell suspension, 24 h immobilization time and pH 6. The change in protein concentration of free and immobilized cells was studied during a full biodegradation cycle. The rate of phenol biodegradation by T. cutaneum R57 cells immobilized on porous polyacrylonitrile (PAN) fibres was higher than that obtained using modified PAN beds.
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