Abstract
In the construction of biosensors incorporating probe oxygen electrodes as the biochemical signal transducer, acetylcellulose membranes have proved to be a popular but poorly characterized matrix for microbial cell immobilization. In this report the relationship between immobilized whole microbial cell concentration/mm3 of acetylcellulose membrane and the reduction in oxygen diffusion across the microbial electrode has been determined using enteric bacteria. Total inhibition of oxygen diffusion occurred when the cell concentration was raised from 1×106 to 1×107 cells mm3: equivalent to a microbial cell volume of 1.5% compared to the maximum theoretical matrix volume. To facilitate future biosensor design a biosensor immobilized cell concentration “Bicc” value and a zero oxygen diffusion “ZO2D” value are suggested, and the parameters of immobilized cell concentration, reduction in oxygen diffusion by immobilized cells and microbial cell size are discussed.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
