Cell expansion in the epidermis: microtubules, cellulose orientation and wall loosening enzymes

Abstract

Two models of isolated epidermis were used to demonstrate that the net orientation of cellulose microfibrils in the cell wall is related to mechanical properties of the tissue, and can be used as an indicator for wall anisotropy. In the developing plant epidermis, cells expand in one or two directions in the plane of the plant surface. In epidermis cells actively expanding in one direction (elongation), the orientation of cortical microtubules closely matches the net cellulose orientation. In epidermis cells expanding in two directions, the orientation of the parallel microtubules does not coincide with the net cellulose orientation in the adjacent cell wall. The orientation of cortical microtubules is thus not always a reliable indicator of wall characteristics. In both types of epidermis, a high rate of expansion correlates with a high activity of xyloglucan endotransglycosylase (XET), as determined in situ . This high activity alone cannot explain unidirectional wall expansion.