Abstract
Syrian hamster embryo (SHE) cells offer the possibility to study the direct relationship between gap junctional intercellular communication (GJIC) and cell transformation. SHE cells express connexin43 and inhibition of communication is paralleled by a decrease in the number of connexon structures, while connexin43 mRNA levels are essentially unchanged. For several compounds, such as phorbol esters, cAMP modulators, retinoids and inhibitors of protein kinase C (pkC), the data indicate a role for decreased communication in colony morphological transformation, since enhancement of transformation is accompanied by a decrease in GJIC. For other compounds, such as DDT, glucocorticoids and vanadate, no such relationship was observed. DDT decreased communication with little or no effect on transformation, glucocorticoids blocked transformation with no effect on communication, and vanadate induced morphological transformation with no effect on communication. The results suggest the existence of posttranslational mechanisms in the regulation of connexin function. Although altered GJIC may be involved in the transformation of SHE cells by certain compounds, decreased communication is neither sufficient, nor necessary for the induction of transformed morphology in SHE cells.
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