Abstract
We examined distribution, expression and hormonal regulation of specific gap junction proteins in four osteoblastic cell lines as well as osteoblastic cells isolated from young (4 month) and old (24 month) rats. Cx43 was found to be the dominant gap junction protein expressed in all the cell types we examined. Parathyroid hormone stimulated functional coupling in osteoblastic cells isolated from young rats but not in the osteoblastic cell lines or osteoblastic cells isolated from old rats. Furthermore, Cx43 protein abundance was reduced in osteoblastic cells isolated from old rats.
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