Abstract
BackgroundThe aim of this study is to verify the feasibility of using silk fibroin (SF) as a potential membrane for guided bone regeneration (GBR).MethodsVarious cellular responses (i.e., cell attachment, viability, and proliferation) of osteoblast-like MG63 cells cultured on an SF membrane were quantified. After culturing on an SF membrane for 1, 5, and 7 days, the attachment and surface morphology of MG63 cells were examined by optical and scanning electron microscopy (SEM), cell viability was determined using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and cell proliferation was quantified using 4′,6-diamidino-2-phenylindole (DAPI) fluorescence staining.ResultsOptical microscopy revealed that MG63 cells cultured on the SF membrane proliferated over the 7-day observation period. The viability of cells cultured on SF membranes (SF group) and on control surfaces (control group) increased over time (P < 0.05); however, at respective time points, cell viability was not significantly different between the two groups (P > 0.05). In contrast, cell proliferation was significantly higher in the SF membrane group than in the control group at 7 days (P < 0.05).ConclusionsThese results suggest that silk fibroin is a biocompatible material that could be used as a suitable alternative barrier membrane for GBR.
Highlights
The aim of this study is to verify the feasibility of using silk fibroin (SF) as a potential membrane for guided bone regeneration (GBR)
MTT assay for cell viability The viability of cells cultured on SF membranes for various times up to 7 days was evaluated using thiazolyl blue tetrazolium bromide (MTT, Sigma-Aldrich, Inc., USA) following the manufacturer’s instructions
Cell attachment and proliferation on SF membranes MG63 cells were seeded onto SF membranes (SF membrane group) or on culture plastic and assessed for differences in their proliferation over a range of 1 to 7 days (Fig. 2)
Summary
The aim of this study is to verify the feasibility of using silk fibroin (SF) as a potential membrane for guided bone regeneration (GBR). Silk fibroin (SF) has the ability to support the growth of different cell types, including endothelial, epithelial, Yoo et al Maxillofacial Plastic and Reconstructive Surgery (2016) 38:17 fibroblast, glial, keratinocyte, and osteoblast cells [14]. It has been applied in various biomedical applications: as a substrate material for tissue engineering scaffolds, in drug delivery, and even as artificial blood vessels because of its high tensile strength and low solubility in aqueous solutions [15, 16]. The physical and biological properties of silk fibroin make it an ideal candidate material for barrier membranes that resolve the limitations of previously developed membranes [17, 18]
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