Abstract
The main objective of this work is to synthesize CdTe quantum dots (QDs) conjugated with Concanavalin A (Con A) as a novel biosensor to be selective and specific for the detection of Lipopolysaccharide (LPS). In addition, the conjugated CdTe QDs–Con A was used as fluorescence labels to capture Serratia marcescens bacteria through the recognition between CdTe QDs–Con A and LPS of S. marcescens. The appearance of the lattice plans in the high resolution transmission electron photograph indicated a high crystalline with an average size of 4–5nm for the CdTe QDs. The results showed that the relative fluorescence intensity of CdTe QDs–Con A decreased linearly with LPS concentration in the range from 10 to 90fg/mL and with correlation coefficient (R2) equal to 0.9713. LPS surrounding the S. marcescens bacteria was bound to the CdTe QDs–Con A and leads to quenching of PL intensity. It was found that a good linear relationship between the relative PL intensity and the logarithmic of cell population of S. marcescens in range from 1×10 to 1×106CFU/mL at pH 7 with R2 of 0.952 was established.
Published Version
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call