Abstract
The fungal pathogenBipolaris maydis invades by direct penetration into maize leaf veins. In order to understand the resistance mechanism of maize toB. maydis strain 523, cDNA-AFLP (amplified fragment length polymorphism) analysis was conducted to compare the changes in mRNA transcripts in response toB. maydis infection between a highly disease-resistant (HDR) line and a susceptible (S) line. 13 cDNA fragments derived from the genes showing enhanced expression after fungal infection, namedHDR genes, were isolated from the HDR line. Northern blot analysis showed that 5HDR genes were induced by fungal infection in the HDR, but not the S lines. The 5HDR genes showed homology to previously characterized genes involved in disease resistance. A full-lengthHDR10 cDNA was isolated. It had a capacity to encode a protein of 284 amino acids. The deduced amino acid sequence of theHDR10 gene was homologous to a fungal infection-induced protein fromCicer arietinum and a hypersensitive response protein from maize, respectively. These results suggest that maize resistance toB. maydis infection in the HDR line may be mediated by the induction of multiple defense-related genes.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
