Abstract
Publisher Summary Cyclin-dependent kinase-3 (Cdk3) that is encoded by cDNA, has been cloned via homology to the Cdc2/Cdc28 family. The CDK3 gene can complement its mutants of Saccharomyces cerevisiae cdc28, suggesting that Cdk3 could be a cell-cycle regulator in vertebrate cells. Dominant negative mutants of cdk3 arrest mammalian cells in G1. Like other Cdks, Cdk3 consists essentially of a catalytic domain only. Cyclins are presumed to act as regulatory subunits, but this has not been demonstrated under physiological conditions. Physiological substrates have not been identified, but transfected Cdk3 can phosphorylate histone H1. The enzyme can be assayed by transfer of radioactivity from [γ32P]ATP to histone HI. CDK3 mRNA and Cdk3 protein are expressed in a broad variety of cell types at very low levels.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
