CBIO-27TUMOR SUPPRESSION EFFECT OF mir-29b IN GLIOBLASTOMA

Abstract

Glioblastoma (GBM) is known as one of the most fatal forms of cancer. MicroRNAs have been widely implicated in the regulation of mammalian development and its pathogenesis. The brain-enriched miR-29 subfamilies are known to be exclusively expressed in the developing brain, while, they are aberrantly down-regulated in brain tumors. Besides, the mechanisms by which miR-29 mediated signal components in brain neoplasms remain undetermined. This study aims to elucidate the role of miR-29b in GBM development and the feasibility of therapeutic target using conjugated nanoparticles. We have discovered that increase in apoptotic cell populations with introduction of miR-29b in A172, a PTEN-deficient GBM cell line. An established human-derived GBM tissue slice culture system confirmed the anticancer effect of the miR-29b-conjugated-nanoparticle. Using PCR array, we found that exogenous miR-29b inhibit SPARC (secreted protein acidic and rich in cysteine) expression which mediates anticancer effect. The suppression of miR-29b regulates SPARC and contributes tumor development in GBM. Introduction of miR-29b using nanoparticle-delivery system can be considered as putative therapeutic strategy in GBM.