CBIO-01. THE EARLY CHANGES IN PROTEIN EXPRESSION IN THE RHESUS OPTIC NERVES INJURED BY A SINGLE DOSE/FRACTIONATION STEREOTACTIC RADIOSURGERY

Abstract

Radiation-induced optic neuropathy (RION) is a severe complication when we use stereotactic radiosurgery (SRS) to treat anterior visual disease. This study was designed to analyze the early changes in protein expression in rhesus optic nerves injured by SRS. The left side intraorbital optic nerves of 3 rhesus monkeys (6-8 years)were injured by gamma knife surgery (GKS) (marginal dose of 15 Gy, 50% isodose curve), while the contralateral optic nerves served as the control. The bilateral intraorbital optic nerves of 3 rhesus monkeys were dissected and performed a non-marker quantitative proteomic analysis at 72 hours after GKS. The differential expression protein information was obtained. The functional information of those proteins was obtained through the BLAST sequence alignment with the human proteomic. The result showed that 41 proteins fit the criteria for differential expression (change>2.0-fold, P value<0.05 or exclusive expression). 7 proteins were significantly down-regulated (change>2.0-fold, P value<0.05) at the injured optic nerves. 18 proteins were exclusively expressed in the contralateral optic nerves. And 16 proteins were exclusively expressed in the injured optic nerves. In general, this study indicated that the single dose of 15 Gy on a 50% isodose curve in the optic nerve had a significant impact on protein expression at 72 hours after GKS. Non-marker quantitative analysis based on high-resolution LCMS/MS can be used to detect the early protein expression change in RION. At 72h after GKS, The cell’s endocytosis and pinocytosis were declined and shown a significant inflammation and immunization. The proliferation of those cell were declined and the genetic transcription and translation were increased in the injury optic.