Abstract
1. 1. Cation transport in relation to cell metabolism in the high Na +, low K + seal erythrocyte was investigated. Under steady-state conditions at 40°C, Na + efflux was32 ± 4·0 (S.D.) m-equiv. × kg RBC H 2O −1 × hr −1and K + influx was 1·1 ± 0·38m-equiv. × kg RBC H 2O −1 × hr −1. 2. 2. Both fluxes were insensitive to ouabain (10 −4 M) and an inhibitor of anaerobic metabolism, monoiodoacetate (10 −4 M). 3. 3. Ethanol (5 × 10 −1 M) decreased Na + efflux in the seal erythrocyte and the high Na +, low K + cat erythrocyte but did not affect Na + transport in the low Na +, high K + human erythrocyte. 4. 4. Valinomycin (10 −5 M) produced increased permeability to K + without effect on Na + transport. 5. 5. Lactate production (2·4 ± 0·29 mM×1.RBC H 2O −1 × hr −1) was not decreased significantly by ouabain. 6. 6. Adenosinetriphosphate (ATP) concentrations (0·4 ± 0·03 (S.D.)mM/5·0 mM Hb) and 2,3 diphosphoglycerate (2,3 DPG) concentrations (6·5 ± 0·53 (S.D.) mM/5·0 mM Hb) provide evidence that this cell possesses an active glycolytic mechanism for high energy phosphate production. 7. 7. Quantitatively, the level of energy transduction is similar in both high Na +, low K + seal erythrocytes and low Na +, high K + human erythrocytes despite the small calculated minimal work cost of cation transport in the former.
Published Version
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