Abstract
A study has been made of the sugar substrate specificities and the cation specificities of the melibiose transport system of Escherichia coli. The following β-galactosides were found to be transported: lactose, l-arabinose-β- d-galactoside, d-fructose-β- d-galactoside, o- and p- nitrophenyl-β- d-galactosides . These β-galactosides were cotransported with Na + but not with H +. The α-galactosides raffinose, melibiose and p-nitrophenyl- α-galactoside were transported with either H + or Na +. Of the monosaccharides tested d-galactose could use either Na + or H + for cotransport whereas d-fucose, l-arabinose and d-galactosamine could use only Na +. The sugar specificity requirements for H + cotransport are therefore more exacting than those for Na + cotransport.
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Schedule a callMore From: Biochimica et Biophysica Acta (BBA) - Biomembranes
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