Abstract
Introduction: Urinary Tract Infection (UTI) is caused by the abnormal growth of the pathogen in the urinary tract. Urine Microscopy and culture is still the gold standard for the isolation of bacteria. However, screening tests are cost-effective and more practical in managing UTIs. Aim: To determine rapid screening tests (Gram Staining and Catalase test) compared to culture. Materials and Methods: The cross-sectional study was done in the Department of Microbiology, Jawaharlal Nehru Medical College; Acharya Vinoba Bhave Rural Hospital, Sawangi Meghe, Wardha, Maharashtra, India, for a period of one year from August 2019 to September 2020. In this study, 100 urine samples were processed by screening tests such as the Catalase test and Gram stain, followed by culture. Results: Positive predictive value of the catalase test was 55.31% and Gram’s stain was 78.26%. In contrast, the negative predictive value of catalase was 69.81%, and Gram stain was 88.88%. The sensitivity and specificity of the catalase test was 61.90% and 63.79%, respectively and the sensitivity and specificity of Gram stain was 85.71% and 82.75%, respectively. Conclusion: Gram stain had the highest sensitivity, 85.71%, and specificity of 82.75% compared to the catalase test. Candida spp. was the most frequently isolated from a urine culture, followed by Enterococcus spp. E. coli, and Klebsiella spp. were also commonly isolated from people.
Published Version
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