Catalase active metal-organic framework synthesized by ligand regulation for the dual detection of glucose and cysteine

Abstract

Mimic enzymes greatly improve the inherent insufficiencies of natural enzymes. Therefore, mimic enzyme sensors attract increasing research interest. Metal-organic framework (MOF) is emerging in the field of mimic enzyme catalysis due to its remarkable structural properties. In this paper, a colorimetric method is designed for rapid and sensitive detection of glucose and cysteine levels. The MOF Eu-pydc (pydc—2,5-pyridinedicarboxylic acid) is synthesized by a new strategy which is regulated by ligands at room temperature and found to have peroxidase activity. Then, the MOF is used as a mimic enzyme to catalyze chromogenic substrate (3,3′,5,5′-tetramethylbenzidine, TMB) for colorimetric sensing of glucose. The developed method can accurately detect glucose in the range of 10 μM–1 mM (R2 = 0.9958) with a relatively low detection limit about 6.9 μM. Moreover, a cysteine sensor with a detection limit of 0.28 μM is also established based on the disappearance of the color of oxTMB. Additionally, the proposed glucose sensor exhibits excellent selectivity and is successfully applied to blood glucose detection. At the same time, the detection of cysteine is also highly sensitive. In short, the dual sensor is fast, low cost, and convenient, and has great application potential in the diagnosis of disease.