Cassia occidentalis Potentiates wound Healing Process in Type-2 Diabetic Rats

Abstract

Indian Journal of Natural Products,2022,36,1,52-57.DOI:10.5530/ijnp.2022.1.8Published:February 2023Type:Original ArticleAuthors:Rajesh Singh Pawar, Avinash Singh Mandloi, Umesh Kumar Patil, and Shweta Kumar Author(s) affiliations:Rajesh Singh Pawar1,2,*, Avinash Singh Mandloi1, Umesh Kumar Patil3, Shweta Kumar4 1Pharmacognosy and Phytochemistry Laboratory, Faculty of Pharmacy, VNS Group of Institutions, Bhopal, Madhya Pradesh, INDIA. 2Department of Pharmacy, Truba Institute of Pharmacy, Bhopal, Madhya Pradesh, INDIA. 3Department of Pharmacognosy, Department of Pharmaceutical sciences, Dr. Harisingh Gour Vishwavidyalaya (A Central University), Sagar, Madhya Pradesh, INDIA. 4Department of General Medicine, All India Institute of Medical Sciences, Bhopal, Madhya Pradesh, INDIA. Abstract:Introduction: This work is focus on a cost-effective herbal hydrogel formulation comprising of extract, from the leaves of Cassia occidentalis L. (Caesalpiniaceae). The formulation is highly effective for delayed wound healing due to diabetes. Background: Cassia occidentalis (CO) is extensively used in folklore medicine system. It is a common weed scattered from foothills of Himalayas to West Bengal, South India, Burma and Srilanka. Objectives: The study aimed to explore the role of Cassia occidentalis (leaf) for wound healing potential in diabetic rats using the excision wound model. Materials and Methods: The characterization of the phytoconstituents and bioavailability profiles of methanolic extract of CO (MCO) were investigated using High-Performance Liquid Chromatography-Photodiode Array Detector analysis. Insulin resistance was induced in rats by feeding them a diet rich in fructose and hyperglycemia was induced by injecting streptozotocin (STZ) at a dose of 40 mg/kg body weight (bw), intraperitoneally (ip). After confirmation of diabetes fasting blood glucose level, the wound was induced. Excision wound healing activity was examined on Wistar rats. Results: The hydrogel of MCO (5 and 10%) reduced significantly wound size. Significant mean epithelialization period, higher hydroxyproline content and tensile strength were revealed at the concentrations of MCO hydrogel of 5 and 10% compared to the diabetic wound control group. Conclusion: These results provide promising baseline information for the wound healing potential of MCO in type-2 diabetes treatment. Keywords:Biochemical parameters, Cassia occidentalis, Hydrogel, Hydroxyproline, HyperglycemiaView:PDF (906.38 KB)