Casein fortified diet reversed the diabetes like changes induced by 4G connected mobile phone radiation in mice

Abstract

Aim: Mobile phone usage is increasing day by day and has become an indispensable part of our life. However, people are constantly being exposed to the harmful electromagnetic radiation emitted from mobile phones and the remedy for the same is yet indiscernible. In our current report, we have investigated the role of a high casein diet (HCD) in ameliorating the pancreatic and hepatotoxic effects of mobile phone radiation. Materials and Methods: Mice (n=24) were divided into four groups and maintained as control groups (Gr-A: normal diet; Gr-D: HCD) and mobile phone radiation (SAR value: 1.5 W/ kg at 1g body wt.) exposed groups, irradiated for 3 hours/ day for 90 days (Gr-B: normal diet; Gr-C: HCD). The normal diet contained 5% casein while HCD contained 20% casein. After the tenure of radiation exposure terminates, animals were sacrificed; the pancreatic, hepatic tissues, and blood were collected for the histology and serum isolation respectively. Periodic acid- Schiff staining was performed to assess the glycogen content of hepatic tissue and hematoxylin-eosin staining for general histological analysis. The serum was used to estimate the fasting glucose level. Observations: Increased fasting serum glucose level and shrunken pancreatic islets on radiation exposure may indicate the diabetes progression. Loss of glycogen storage capacity by the liver was observed in radiation exposed group which are suggestive of compromised hepatic functions. Histo-architectural analysis of the liver revealed severely distorted hepatic cells with prominent occurrences of karyorrhexis and karyolysis, irregular and enlarged sinusoidal spaces upon radiation exposure. HCD countermanded these abnormal manifestations of the electromagnetic radiation to a considerable extent. Inference: Thus, electromagnetic radiation from the mobile phone may be responsible for the impairment of glucose metabolism and thereby enhancing the progression of diabetes in mice. In contrast, HCD was prudent in combating these degenerative alterations of mobile phone radiation.