Abstract
Muotri, A.R. et al. (2005): Development of functional human embryonic stem cell-derived neurons in mouse brain. Proceedings of the National Academy of Sciences USA 102, pp. 18644-1848 Human embryonic stem cells (hESC) from an already established line (Cyth25) were differentiated to cells of the neuronal lineages both in vitro and in vivo to get insight into human neural differentiation. In vitro differentiation was obtained by co-culture with mouse skull bone marrow-derived stromal cells. Undifferentiated hESC dedicated for transplantation were transfected to express enhanced green fluorescent protein (EGFP) and transplanted in utero into the brain (lateral ventricle) of mouse embryos at embryonic day 14. The chimeric mice were killed at different ages (two and 18 month) and the brain analysed via immunofluorescence and electrophysiology methods.
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