Abstract
Robust and efficient multimodal catalysis platforms that possess high catalytic activity and enhanced electrical conductivity are preferred for enhancing the electrical analysis performance under a low overpotential. This report describes a function-switchable amperometric sensor for electrochemical monitoring hydrogen peroxide and glucose under relatively low overpotential based on PEI-functionalized metal–organic framework (P-MOF) supported gold nanoparticles/nitrogen-doped graphene quantum dots (AuNPs/N-GQDs) and glucose oxidase. AuNPs/N-GQDs with high peroxidase mimicking activity was anchored on the P-MOF modified electrode surface as H2O2 sensors, exhibiting a high sensitivity of 134.26 μA mM−1 cm−2 and a detection limit of 3.38 μM. Subsequently, AuNPs/N-GQDs-P-MOF used as a nanocarrier for glucose oxidase (GOx) to achieve “cascade nanoreactor” amplification for glucose detection. The amplified amperometric glucose biosensor showed excellent anti-interference ability, reproducibility, and a limit of detection as low as 0.7 μM (S/N = 3) with high sensitivity of 1512.4 μA mM−1 cm−2. In addition, the feasibility of the electrochemical biosensor for accurate and quantitative detection of glucose in human serum samples was confirmed with a recovery ranging from 93.2 to 99.3%. The strategy of combining AuNPs/N-GQDs-P-MOF and glucose oxidase for cascade catalysis in this work provides a simple yet efficient approach to prepare nanomaterial-based enzyme-mimics and enzymes hybrids with high performance, which holds great potential in biological and chemical applications of electrochemical devices and biosensing.
Published Version
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