CART is expressed in refeeding-activated neurons of the ventral parvocellular part of the hypothalamic paraventricular nucleus

Abstract

Event Abstract Back to Event CART is expressed in refeeding-activated neurons of the ventral parvocellular part of the hypothalamic paraventricular nucleus Tamás Füzesi1*, Gabor Wittmann1, Zsolt Liposits1, Ronald M. Lechan2, 3 and Csaba Fekete1, 2 1 Department of Endocrine Neurobiology, Institute of Experimental Medicine, Hungary 2 Tupper Research Institute and Department of Medicine , New England Medical Center, United States 3 Department of Neuroscience, Tufts University School of Medicine, United States Refeeding after a period of fasting results in satiety and melanocortin-mediated activation of neurons in the ventral parvocellular subdivision of the PVN (vPVN) suggesting that the vPVN is an important component of the melanocortin signaling system. Since the anorexigenic peptide, CART is expressed in vPVN neurons, we hypothesized that CART synthesized by these neurons may contribute to the satiety effects of α-MSH. To test this hypothesis, double-labeling combined immunofluorescence for c-Fos protein, as a marker of neuronal activation, and isotopic in situ hybridization for CART mRNA detection was performed on hypothalamic sections of fasting and refed (2h) rats. The ratio of activated CART neurons was quantified in the vPVN. C-Fos-immunoreactivity was absent in the entire PVN of fasted animals. In contrast, following refeeding, c-Fos-containing neurons were observed in the PVN, including the strongly labeled vPVN. The most intense CART mRNA signal was detected in the medial part of the vPVN, but neurons with less intense CART mRNA expression were also observed throughout the vPVN. Analyses of double-labeled preparations demonstrated that 44 ± 2 % of the activated neurons in vPVN contained CART mRNA, while 58 ± 8 % of CART-synthesizing neurons in this region contained c-Fos-immunoreactivity. These data demonstrate that CART is synthesized in approximately half of the refeeding-activated neurons in the vPVN. Since the vPVN neurons are activated by the melanocortin system, CART may be involved in relaying the satiety effects of melanocortins to downstream target centers. Conference: 12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009. Presentation Type: Poster Presentation Topic: Homeostatic regulatory mechanisms Citation: Füzesi T, Wittmann G, Liposits Z, Lechan RM and Fekete C (2009). CART is expressed in refeeding-activated neurons of the ventral parvocellular part of the hypothalamic paraventricular nucleus. Front. Syst. Neurosci. Conference Abstract: 12th Meeting of the Hungarian Neuroscience Society. doi: 10.3389/conf.neuro.01.2009.04.231 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 13 Mar 2009; Published Online: 13 Mar 2009. * Correspondence: Tamás Füzesi, Department of Endocrine Neurobiology, Institute of Experimental Medicine, Budapest, Hungary, fuzesi@koki.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Tamás Füzesi Gabor Wittmann Zsolt Liposits Ronald M Lechan Csaba Fekete Google Tamás Füzesi Gabor Wittmann Zsolt Liposits Ronald M Lechan Csaba Fekete Google Scholar Tamás Füzesi Gabor Wittmann Zsolt Liposits Ronald M Lechan Csaba Fekete PubMed Tamás Füzesi Gabor Wittmann Zsolt Liposits Ronald M Lechan Csaba Fekete Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.