Abstract

Carbonic anhydrase was detected in the face fly, Musca autumnalis, and the development profile of the enzyme showed that maximum enzymatic activity occurred in the post-feeding larva. By comparison, little carbonic anhydrase activity was observed in post-feeding larvae of the house fly, Musca domestica. The enzyme was localized in the haemolymph, salivary glands, midgut and brain of post-feeding face fly larvae using an electrometric assay and Hansson's histochemical technique. No enzymatic activity was detected in the anterior Malpighian tubules, posterior Malpighian tubules, fat body, tracheae and carcass. These findings suggest that carbonic anhydrase has no function in the formation of the calcospherites located in the anterior Malpighian tubules of post-feeding larvae, but may be important in the ecdysial elimination of minerals during pupariation.

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