Abstract
In the synthesis of carbamoyl phosphate from HCO 3 − and NH3 by carbamoyl phosphate synthetases HCO 3 − is first activated by ATP (ATPA), with formation of enzyme-bound carboxyphosphate and ADP. Mono and divalent cations are required in this reaction. Carboxyphosphate appears also to be formed with biotin-dependent carboxylases and with phosphoenolpyruvate carboxylase. With the carbamoyl phosphate synthetases carboxyphosphate is believed to react with ammonia in a second step, to give enzyme-bound carbamate, which would then be phosphorylated by another molecule of ATP (ATPB). However, a concerted mechanism for the conversion of carboxyphosphate to carbamoyl phosphate has not been ruled out.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
