The Molecular Basis of Substrate Channeling

Abstract

Substrate channeling is the process of direct transfer of an intermediate between the active sites of two enzymes that catalyze sequential reactions in a biosynthetic pathway (for reviews see Refs. 1Srere P.A. Complexes of sequential metabolic enzymes..Annu. Rev. Biochem. 1987; 56: 89-124Crossref PubMed Scopus (807) Google Scholar and 2Ovadi J. Physiological significance of metabolite channeling..J. Theor. Biol. 1991; 152: 1-22Crossref PubMed Scopus (194) Google Scholar). The active sites can be located either on separate domains in a multifunctional enzyme or on separate subunits in a multienzyme complex. Substrate channeling has been proposed to decrease transit time of intermediates, prevent loss of intermediates by diffusion, protect labile intermediates from solvent, and forestall entrance of intermediates into competing metabolic pathways (2Ovadi J. Physiological significance of metabolite channeling..J. Theor. Biol. 1991; 152: 1-22Crossref PubMed Scopus (194) Google Scholar). Loss of an intermediate by diffusion may be especially important in the case of a neutral species, such as indole, which could escape from the cell by passive diffusion across cell membranes (2Ovadi J. Physiological significance of metabolite channeling..J. Theor. Biol. 1991; 152: 1-22Crossref PubMed Scopus (194) Google Scholar, 3Davis B.D. On the importance of being ionized..Arch. Biochem. Biophys. 1958; 78: 497-509Crossref PubMed Scopus (78) Google Scholar, 4Manney T.R. Physiological advantage of the mechanism of the tryptophan synthetase reaction..J. Bacteriol. 1970; 102: 483-488Crossref PubMed Google Scholar, 5Knowles J.R. Calmer waters in the channel..J. Theor. Biol. 1991; 152: 53-56Crossref PubMed Scopus (9) Google Scholar). Nevertheless, there has been considerable debate over whether channeling actually occurs and whether it is advantageous (5Knowles J.R. Calmer waters in the channel..J. Theor. Biol. 1991; 152: 53-56Crossref PubMed Scopus (9) Google Scholar, 6Ovadi J. Physiological significance of metabolite channeling: author's response to commentaries..J. Theor. Biol. 1991; 152: 135-141Crossref PubMed Scopus (23) Google Scholar). X-ray crystallographic studies on several enzyme complexes have revealed two molecular mechanisms for channeling. The discovery of an intramolecular tunnel in tryptophan synthase (7Hyde C.C. Ahmed S.A. Padlan E.A. Miles E.W. Davies D.R. Three-dimensional structure of the tryptophan synthase α2β2 multienzyme complex from Salmonella typhimurium. J. Biol. Chem. 1988; 263: 17857-17871Google Scholar) provided the first molecular mechanism for channeling. For a long time this was a unique example, but recent structure determinations have revealed plausible evidence for tunneling of ammonia and carbamate in carbamoyl-phosphate synthase (CPS) 1The abbreviations used are: CPS, carbamoyl-phosphate synthase; GPATase, glutamine phosphoribosylpyrophosphate amidotransferase; DHFR, dihydrofolate reductase; TS, thymidylate synthase; PRPP, phosphoribopyrophosphate(8Thoden J.B. Holden H.M. Wesenberg G. Raushel F.M. Rayment I. Structure of carbamoyl phosphate synthetase: a journey of 96 Å from substrate to product..Biochemistry. 1997; 36: 6305-6316Crossref PubMed Scopus (306) Google Scholar) and of ammonia in phosphoribosylpyrophosphate amidotransferase (GPATase) (9Krahn J.M. Kim J.H. Burns M.R. Parry R.J. Zalkin H. Smith J.L. Coupled formation of an amidotransferase interdomain ammonia channel and a phosphoribosyltransferase active site..Biochemistry. 1997; 36: 11061-11068Crossref PubMed Scopus (187) Google Scholar). The structure of dihydrofolate reductase-thymidylate synthase, however, showed no evidence for a tunnel between the two active sites (10Knighton D.R. Kan C.C. Howland E. Janson C.A. Hostomska Z. Welsh K.M. Matthews D.A. Structure of and kinetic channeling in bifunctional dihydrofolate reductase-thymidylate synthase..Nat. Struct. Biol. 1994; 1: 186-194Crossref PubMed Scopus (203) Google Scholar). Neither are the two active sites adjacent to one another. Instead, there are positively charged residues along the surface between the active sites that form an electrostatic highway sufficient to channel the negatively charged dihydrofolate with high efficiency (10Knighton D.R. Kan C.C. Howland E. Janson C.A. Hostomska Z. Welsh K.M. Matthews D.A. Structure of and kinetic channeling in bifunctional dihydrofolate reductase-thymidylate synthase..Nat. Struct. Biol. 1994; 1: 186-194Crossref PubMed Scopus (203) Google Scholar, 11Stroud R.M. An electrostatic highway..Nat. Struct. Biol. 1994; 1: 131-134Crossref PubMed Scopus (47) Google Scholar). The focus of this minireview will be on the structural basis of channeling in the four enzyme structures cited above and on solution studies of these systems. 2We will not consider enzymes in which the intermediate is covalently bound (e.g. pyruvate dehydrogenase and α-ketoglutarate dehydrogenase).Scheme FSI summarizes the reactions catalyzed by the four enzymes. A comparison of the structural and kinetic results reveals that these enzymes frequently exhibit allosteric interactions that synchronize the reactions to prevent the build-up of excess intermediate (12Anderson K.S. Miles E.W. Johnson K.A. Serine modulates substrate channeling in tryptophan synthase: a novel intersubunit triggering mechanism..J. Biol. Chem. 1991; 266: 8020-8033Abstract Full Text PDF PubMed Google Scholar, 13Kim J.H. Krahn J.M. Tomchick D.R. Smith J.L. Zalkin H. Structure and function of the glutamine phosphoribosylpyrophosphate amidotransferase glutamine site and communication with the phosphoribosylpyrophosphate site..J. Biol. Chem. 1996; 271: 15549-15557Crossref PubMed Scopus (81) Google Scholar, 14Pan P. Woehl E. Dunn M.F. Protein architecture, dynamics and allostery in tryptophan synthase channeling..Trends Biochem. Sci. 1997; 22: 22-27Abstract Full Text PDF PubMed Scopus (93) Google Scholar, 15Liang P.H. Anderson K.S. Substrate channeling and domain-domain interactions in bifunctional thymidylate synthase-dihydrofolate reductase..Biochemistry. 1998; 37: 12195-12205Crossref PubMed Scopus (59) Google Scholar, 16Miles B.W. Banzon J.A. Raushel F.M. Regulatory control of the amidotransferase domain of carbamoyl phosphate synthetase..Biochemistry. 1998; 37: 16773-16779Crossref PubMed Scopus (34) Google Scholar). Tryptophan synthase catalyzes the last two reactions in the biosynthesis of l-tryptophan (SchemeFSI, A) (for reviews see Refs. 14Pan P. Woehl E. Dunn M.F. Protein architecture, dynamics and allostery in tryptophan synthase channeling..Trends Biochem. Sci. 1997; 22: 22-27Abstract Full Text PDF PubMed Scopus (93) Google Scholar and 17Yanofsky C. Crawford I.P. Boyer P.D. The Enzymes. 7. Academic Press, New York1972: 1-31Google Scholar, 18Miles E.W. Structural basis for catalysis by tryptophan synthase..Adv. Enzymol. Relat. Areas Mol. Biol. 1991; 64: 93-172PubMed Google Scholar, 19Miles E.W. Biswas B.B. Roy S. Subcellular Biochemistry, Proteins: Structure, Function, and Protein Engineering. 24. Plenum Press, New York1995: 207-254Google Scholar). In bacteria, the two reactions are catalyzed by separate α and β subunits, which combine to form a stable multienzyme complex, (αβ)2. In yeast and molds, the two reactions are catalyzed by separate α and β sites in a single bifunctional polypeptide chain, (α-β)2. 3We use the hyphen to indicate that two different catalytic domains (e.g. α and β) are located on a single polypeptide chain in a bifunctional or multifunctional enzyme.Evidence that indole is not liberated as a free intermediate in the overall conversion of indole-3-glycerol phosphate andl-serine to l-tryptophan suggested that indole passes directly from the α site to the β site without release to the surrounding solvent (20Yanofsky C. Rachmeler M. The exclusion of free indole as an intermediate in the biosynthesis of tryptophan in Neurospora crassa..Biochim. Biophys. Acta. 1958; 28: 641-642Crossref Scopus (53) Google Scholar, 21DeMoss J.A. Studies on the mechanism of the tryptophan synthetase reaction..Biochim. Biophys. Acta. 1962; 62: 279-293Crossref PubMed Scopus (54) Google Scholar, 22Creighton T.E. A steady-state kinetic investigation of the reaction mechanism of the tryptophan synthetase of Escherichia coli. Eur. J. Biochem. 1970; 13: 1-10Google Scholar, 23Matchett W.H. Indole channeling by tryptophan synthase of Neurospora..J. Biol. Chem. 1974; 249: 4041-4049Abstract Full Text PDF PubMed Google Scholar). The 2.8-Å resolution crystal structure of tryptophan synthase from Salmonella typhimuriumshowed that the active sites of the α and β subunits are about 25 Å apart (7Hyde C.C. Ahmed S.A. Padlan E.A. Miles E.W. Davies D.R. Three-dimensional structure of the tryptophan synthase α2β2 multienzyme complex from Salmonella typhimurium. J. Biol. Chem. 1988; 263: 17857-17871Google Scholar). The structure also revealed that the α and β sites are connected by a largely hydrophobic intramolecular tunnel with dimensions sufficient to accommodate up to four molecules of indole (Fig. 1). Further refinement of the native structure and determination of several structures in the presence of different ligands have revealed a number of ligand-induced conformational changes that may be important for channeling indole and also for allosteric communication between the α and β subunits (24Rhee S. Parris K.D. Ahmed S.A. Miles E.W. Davies D.R. Exchange of K+ or Cs+ for Na+ induces local and long-range changes in the three-dimensional structure of the tryptophan synthase α2β2 complex..Biochemistry. 1996; 35: 4211-4221Crossref PubMed Scopus (137) Google Scholar, 25Rhee S. Parris K.D. Hyde C.C. Ahmed S.A. Miles E.W. Davies D.R. Crystal structures of a mutant (βK87T) tryptophan synthase α2β2 complex with ligands bound to the active sites of the α and β subunits reveal ligand-induced conformational changes..Biochemistry. 1997; 36: 7664-7680Crossref PubMed Scopus (143) Google Scholar, 26Rhee S. Miles E.W. Davies D.R. Cryo-crystallography of a true substrate, indole-3-glycerol phosphate, bound to a mutant (αD60N) tryptophan synthase α2β2 complex reveals the correct orientation of active site αGlu49..J. Biol. Chem. 1998; 273: 8553-8555Abstract Full Text Full Text PDF PubMed Scopus (51) Google Scholar, 27Rhee S. Miles E.W. Mozzarelli A. Davies D.R. Cryo-crystallography and microspectrophotometry of a mutant (αD60N) tryptophan synthase α2β2 complex reveals allosteric roles of αAsp-60..Biochemistry. 1998; 37: 10653-10659Crossref PubMed Scopus (40) Google Scholar, 28Schneider T.R. Gerhardt E. Lee M. Liang P.-H. Anderson K.S. Schlichting I. Loop closure and intersubunit communication in tryptophan synthase..Biochemistry. 1998; 37: 5394-5406Crossref PubMed Scopus (134) Google Scholar). In the absence of ligands, the active sites of both the α and β subunits are accessible to solvent. Dramatic conformational changes occur upon addition of ligands that bind to both α and β subunits (25Rhee S. Parris K.D. Hyde C.C. Ahmed S.A. Miles E.W. Davies D.R. Crystal structures of a mutant (βK87T) tryptophan synthase α2β2 complex with ligands bound to the active sites of the α and β subunits reveal ligand-induced conformational changes..Biochemistry. 1997; 36: 7664-7680Crossref PubMed Scopus (143) Google Scholar, 28Schneider T.R. Gerhardt E. Lee M. Liang P.-H. Anderson K.S. Schlichting I. Loop closure and intersubunit communication in tryptophan synthase..Biochemistry. 1998; 37: 5394-5406Crossref PubMed Scopus (134) Google Scholar). These changes include ordering of disordered loop structures in the vicinity of the active site of the α subunit together with movement of a β subunit subdomain (residues 93–189). These conformational changes restrict the access of solvent to the two active sites and to the tunnel and may also prevent escape of the indole intermediate into bulk solvent (14Pan P. Woehl E. Dunn M.F. Protein architecture, dynamics and allostery in tryptophan synthase channeling..Trends Biochem. Sci. 1997; 22: 22-27Abstract Full Text PDF PubMed Scopus (93) Google Scholar, 25Rhee S. Parris K.D. Hyde C.C. Ahmed S.A. Miles E.W. Davies D.R. Crystal structures of a mutant (βK87T) tryptophan synthase α2β2 complex with ligands bound to the active sites of the α and β subunits reveal ligand-induced conformational changes..Biochemistry. 1997; 36: 7664-7680Crossref PubMed Scopus (143) Google Scholar, 28Schneider T.R. Gerhardt E. Lee M. Liang P.-H. Anderson K.S. Schlichting I. Loop closure and intersubunit communication in tryptophan synthase..Biochemistry. 1998; 37: 5394-5406Crossref PubMed Scopus (134) Google Scholar). High resolution studies of the native enzyme showed that the tunnel was blocked by the side chain of βPhe-280. However, crystal structures of the enzyme in the presence of different cations showed that exchange of K+ or Cs+ for Na+ results in movement of the side chain of βPhe-280 out of the tunnel (24Rhee S. Parris K.D. Ahmed S.A. Miles E.W. Davies D.R. Exchange of K+ or Cs+ for Na+ induces local and long-range changes in the three-dimensional structure of the tryptophan synthase α2β2 complex..Biochemistry. 1996; 35: 4211-4221Crossref PubMed Scopus (137) Google Scholar), suggesting that βPhe-280 can act as a gate to control passage through the tunnel. This change in structure is accompanied by movements of many tunnel-associated residues that link the α and β active sites. Kinetic studies have established that the tunnel actually functions to channel indole between the two sites (12Anderson K.S. Miles E.W. Johnson K.A. Serine modulates substrate channeling in tryptophan synthase: a novel intersubunit triggering mechanism..J. Biol. Chem. 1991; 266: 8020-8033Abstract Full Text PDF PubMed Google Scholar, 29Dunn M.F. Aguilar V. Brzovic' P.S. Drewe W.F.J. Houben K.F. Leja C.A. Roy M. The tryptophan synthase bienzyme complex transfers indole between the α- and β-sites via a 25–30 Å long tunnel..Biochemistry. 1990; 29: 8598-8607Crossref PubMed Scopus (155) Google Scholar, 30Lane A.N. Kirschner K. Mechanism of the physiological reaction catalyzed by tryptophan synthase from Escherichia coli. Biochemistry. 1991; 30: 479-484Google Scholar). Transient kinetic analysis shows that the rate of indole transfer through the tunnel is very rapid (>1000 s−1), that the reaction of indole at the β site is fast and irreversible, and that the reaction ofl-serine at the β site to form aminoacrylate increases the α site cleavage of indole-3-glycerol phosphate by approximately 30-fold (12Anderson K.S. Miles E.W. Johnson K.A. Serine modulates substrate channeling in tryptophan synthase: a novel intersubunit triggering mechanism..J. Biol. Chem. 1991; 266: 8020-8033Abstract Full Text PDF PubMed Google Scholar). These three features of the reaction kinetics promote efficient channeling of indole and prevent accumulation of the indole intermediate. However, a mutation (βE109D) that reduces the rate of reaction of indole at the β site (12Anderson K.S. Miles E.W. Johnson K.A. Serine modulates substrate channeling in tryptophan synthase: a novel intersubunit triggering mechanism..J. Biol. Chem. 1991; 266: 8020-8033Abstract Full Text PDF PubMed Google Scholar) and another mutation (βC170F or βC170W) that partially blocks the indole tunnel (31Anderson K.S. Kim A.Y. Quillen J.M. Sayers E. Yang X.-J. Miles E.W. Kinetic characterization of channel impaired mutants of tryptophan synthase..J. Biol. Chem. 1995; 270: 29936-29944Crossref PubMed Scopus (35) Google Scholar) result in the accumulation of indole. The kinetic results also indicate that intersubunit communication keeps the reactions catalyzed by the wild-type α and β subunits in phase so that the indole intermediate does not accumulate (12Anderson K.S. Miles E.W. Johnson K.A. Serine modulates substrate channeling in tryptophan synthase: a novel intersubunit triggering mechanism..J. Biol. Chem. 1991; 266: 8020-8033Abstract Full Text PDF PubMed Google Scholar). These kinetic results and others (32Brzovic' P.S. Ngo K. Dunn M.F. Allosteric interactions coordinate catalytic activity between successive metabolic enzymes in the tryptophan synthase bienzyme complex..Biochemistry. 1992; 31: 3831-3839Crossref PubMed Scopus (98) Google Scholar, 33Banik U. Zhu D.-M. Chock P.B. Miles E.W. The tryptophan synthase α2β2 complex: kinetic studies with a mutant enzyme (βK87T) provide evidence for allosteric activation by an aminoacrylate intermediate..Biochemistry. 1995; 34: 12704-12711Crossref PubMed Scopus (24) Google Scholar) argue that the reaction ofl-serine at the β site to form a pyridoxal phosphate-aminoacrylate intermediate produces a conformational change that is transmitted to the α site and enhances the rate of indole formation from indole-3-glycerol phosphate. Thus, indole is produced at the α site with a significant rate only when the β site is ready to receive it. The crystallographic data suggest mechanisms for the allosteric interactions between the two active sites. The ligand-induced closure and ordering of the α subunit loop 6 is accompanied by enhanced interactions with α loop 2. Specifically, interactions between the loop 6 residue, αThr-183, and the loop 2 residue, αAsp-60, as well as main chain interactions decrease the flexibility of loop 2 and are accompanied by increased interactions of loop 2 with residues in the β subunit mobile subdomain (residues 93–189) (25Rhee S. Parris K.D. Hyde C.C. Ahmed S.A. Miles E.W. Davies D.R. Crystal structures of a mutant (βK87T) tryptophan synthase α2β2 complex with ligands bound to the active sites of the α and β subunits reveal ligand-induced conformational changes..Biochemistry. 1997; 36: 7664-7680Crossref PubMed Scopus (143) Google Scholar, 28Schneider T.R. Gerhardt E. Lee M. Liang P.-H. Anderson K.S. Schlichting I. Loop closure and intersubunit communication in tryptophan synthase..Biochemistry. 1998; 37: 5394-5406Crossref PubMed Scopus (134) Google Scholar). These interactions could provide a route of communication from the α site to the β site and vice versa. The combined structural and kinetic results provide a model for the allosteric properties of tryptophan synthase (14Pan P. Woehl E. Dunn M.F. Protein architecture, dynamics and allostery in tryptophan synthase channeling..Trends Biochem. Sci. 1997; 22: 22-27Abstract Full Text PDF PubMed Scopus (93) Google Scholar, 32Brzovic' P.S. Ngo K. Dunn M.F. Allosteric interactions coordinate catalytic activity between successive metabolic enzymes in the tryptophan synthase bienzyme complex..Biochemistry. 1992; 31: 3831-3839Crossref PubMed Scopus (98) Google Scholar). Allosteric signals derived from covalent transformations at the α and β sites are proposed to switch the enzyme from an open, low activity state to which ligands bind to a closed high activity state, which prevents the escape of indole. A remarkable example of channeling occurs in the case of CPS, which catalyzes the synthesis of carbamoyl phosphate from bicarbonate, glutamine, and two molecules of ATP (Scheme FSI,B) (for recent reviews see Refs. 34Zalkin H. Smith J.L. Enzymes utilizing glutamine as an amide donor..Adv. Enzymol. Relat. Areas Mol. Biol. 1998; 72: 87-144PubMed Google Scholar, 35Raushel F.M. Thoden J.B. Reinhart G.D. Holden H.M. Carbamoyl phosphate synthetase: a crooked path from substrates to products..Curr. Opin. Chem. Biol. 1998; 2: 624-632Crossref PubMed Scopus (26) Google Scholar, 36Holden H.M. Thoden J.B. Raushel F.M. Carbamoyl phosphate synthetase: a tunnel runs through it..Curr. Opin. Struct. Biol. 1998; 8: 679-685Crossref PubMed Scopus (40) Google Scholar). Earlier solution studies (37Anderson P.M. Meister A. Bicarbonate-dependent cleavage of adenosine triphosphate and other reactions catalyzed by Escherichia coli carbamyl phosphate synthetase..Biochemistry. 1966; 5: 3157-3163Crossref PubMed Scopus (93) Google Scholar) provided evidence that the synthesis involves a series of four separate reactions that generate three reactive and unstable intermediates: NH3, carboxyphosphate, and carbamate (SchemeFSI, B). These intermediates must be efficiently channeled and the reactions effectively coupled because the reaction stoichiometry is precisely 2 mol of ADP and 1 mol of glutamate for every mole of carbamoyl phosphate (37Anderson P.M. Meister A. Bicarbonate-dependent cleavage of adenosine triphosphate and other reactions catalyzed by Escherichia coli carbamyl phosphate synthetase..Biochemistry. 1966; 5: 3157-3163Crossref PubMed Scopus (93) Google Scholar). The NH3 formed from glutamine must be channeled because the K m for free NH3 is 3 orders of magnitude greater than that of glutamine. In addition, recent results using 13C NMR to measure isotopic oxygen exchange are consistent with a mechanism that requires channeling of the carbamate intermediate (38Raushel F.M. Mullins L.S. Gibson G.E. A stringent test for the nucleotide switch mechanism of carbamoyl phosphate synthetase..Biochemistry. 1998; 37: 10272-10278Crossref PubMed Scopus (17) Google Scholar). CPS from Escherichia coli is composed of two subunits: a small monofunctional glutamine amidotransferase (α), which belongs to the Triad class, one of the two classes of amidotransferases (34Zalkin H. Smith J.L. Enzymes utilizing glutamine as an amide donor..Adv. Enzymol. Relat. Areas Mol. Biol. 1998; 72: 87-144PubMed Google Scholar), and a large, bifunctional subunit (β) in which the N- and C-terminal halves are homologous, each having an ATP binding site. The N-terminal half (βN) catalyzes the production of carboxyphosphate, which reacts with NH3 to form carbamate, whereas the C-terminal half (βC) catalyzes the phosphorylation of carbamate. The 2.8-Å resolution crystal structure 4Currently at 2.1-Å resolution (50Thoden J.B. Raushel F.M. Benning M.M. Rayment I. Holden H.M. The structure of carbamoyl phosphate synthetase determined to 2.1 Å resolution..Acta Crystallogr. Sec. D. 1999; 55: 8-24Crossref PubMed Scopus (86) Google Scholar). (Fig.2) of the enzyme in the presence of ADP, Mn2+, phosphate, and ornithine (an allosteric effector) (8Thoden J.B. Holden H.M. Wesenberg G. Raushel F.M. Rayment I. Structure of carbamoyl phosphate synthetase: a journey of 96 Å from substrate to product..Biochemistry. 1997; 36: 6305-6316Crossref PubMed Scopus (306) Google Scholar,35Raushel F.M. Thoden J.B. Reinhart G.D. Holden H.M. Carbamoyl phosphate synthetase: a crooked path from substrates to products..Curr. Opin. Chem. Biol. 1998; 2: 624-632Crossref PubMed Scopus (26) Google Scholar) revealed that the three active sites are very far apart; the glutamine binding site of the small subunit (α) is 45 Å from the ADP binding site of the carboxyphosphate domain (βN), which is 35 Å from the ADP site in the carbamoyl phosphate domain (βC). Examination of the structure reveals a tunnel with a length of at least 96 Å by which intermediates might pass from the active site of the small subunit to the carbamoyl phosphate site (Fig.2). Although the average minimum radius (3.2–3.5 Å) of the proposed tunnel is sufficient for passage of the NH3 and carbamate intermediates, constrictions (2.1–2.5 Å) are observed in at least two places. Movements of residues in the tunnel wall or ligand-induced conformational changes may enlarge the dimensions of the tunnel. Interestingly, the proposed NH3 tunnel between the glutamine and carboxyphosphate sites is lined with hydrophilic residues and is thus quite different from the hydrophobic NH3 tunnel in GPATase. In contrast, in the proposed tunnel between the carboxyphosphate and carbamoyl phosphate sites, there are few charged side chains that might hydrolyze the labile carbamate intermediate. The coordination of the three active sites of CPS to work synchronously in the synthesis of carbamoyl phosphate must result from allosteric communication between these distant sites (16Miles B.W. Banzon J.A. Raushel F.M. Regulatory control of the amidotransferase domain of carbamoyl phosphate synthetase..Biochemistry. 1998; 37: 16773-16779Crossref PubMed Scopus (34) Google Scholar). However, the mechanism of communication has yet to be determined. CPS catalyzes a very slow hydrolysis of glutamine in the absence of bicarbonate and ATP. Addition of these substrates increases the rate of glutamine hydrolysis by 3 orders of magnitude. GPATase catalyzes the transfer of the amide nitrogen from glutamine to PRPP, producing phosphoribosylamine, pyrophosphate, and glutamate (Scheme FSI, C) (for a recent review, see Ref.34Zalkin H. Smith J.L. Enzymes utilizing glutamine as an amide donor..Adv. Enzymol. Relat. Areas Mol. Biol. 1998; 72: 87-144PubMed Google Scholar). GPATase from E. coli is a bifunctional enzyme that has two separate catalytic domains on a single polypeptide chain: an N-terminal glutaminase domain (α) and a C-terminal acceptor domain (β). The glutaminase domain belongs to a second class of amidotransferases (Ntn) that have a catalytic N-terminal nucleophile. The enzyme is a homotetramer, (α-β)4, and is regulated by nucleotides and by allosteric control. GPATase can utilize either glutamine or free NH3 for the biosynthesis of phosphoribosylamine (Scheme FSI, C). Early studies provided evidence that glutamine and free NH3 bind at distinct sites (39Messenger L.J. Zalkin H. Glutamine phosphoribosylpyrophosphate amidotransferase from Escherichia coli. Purification and properties..J. Biol. Chem. 1979; 254: 3382-3392Abstract Full Text PDF PubMed Google Scholar). First, free NH3 inhibits glutamine hydrolysis and competes with glutamine as an ammonia source. Second, alkylation of an essential catalytic residue by a glutamine analog or by a sulfhydryl reagent inhibited the glutamine-dependent activity but not the NH3-dependent activity. These results support a mechanism for glutamine utilization that involves amide transfer (i.e. channeling) to the NH3 site. Crystal structures have been reported for GPATase from E. coli in an open, inactive form (13Kim J.H. Krahn J.M. Tomchick D.R. Smith J.L. Zalkin H. Structure and function of the glutamine phosphoribosylpyrophosphate amidotransferase glutamine site and communication with the phosphoribosylpyrophosphate site..J. Biol. Chem. 1996; 271: 15549-15557Crossref PubMed Scopus (81) Google Scholar, 40Muchmore C.R. Krahn J.M. Kim J.H. Zalkin H. Smith J.L. Crystal structure of glutamine phosphoribosylpyrophosphate amidotransferase from Escherichia coli. Protein Sci. 1998; 7: 39-51Google Scholar) and in a closed, active form containing analogs of glutamine and PRPP at the two active sites (9Krahn J.M. Kim J.H. Burns M.R. Parry R.J. Zalkin H. Smith J.L. Coupled formation of an amidotransferase interdomain ammonia channel and a phosphoribosyltransferase active site..Biochemistry. 1997; 36: 11061-11068Crossref PubMed Scopus (187) Google Scholar) (Fig. 3). Comparison of these structures reveals that activation of GPATase results in the formation of a 20-Å tunnel that connects the two active sites (9Krahn J.M. Kim J.H. Burns M.R. Parry R.J. Zalkin H. Smith J.L. Coupled formation of an amidotransferase interdomain ammonia channel and a phosphoribosyltransferase active site..Biochemistry. 1997; 36: 11061-11068Crossref PubMed Scopus (187) Google Scholar). This tunnel is created by the ordering of a flexible loop in the C-terminal domain, which closes over the space between the active sites, effectively sequestering both sites from bulk solvent. This change is accompanied by the kinking of the adjacent C-terminal α-helix. In contrast to CPS, the walls of the tunnel are lined with conserved hydrophobic residues. The hydrophobic tunnel may function not only as a passageway for NH3 but also to exclude water and ensure that NH3 is the only nucleophile able to enter the tunnel and to react with PRPP. The conformational differences between the active and inactive forms provide a structural basis for understanding how PRPP binding in the acceptor domain stimulates glutaminase activity in the glutamine domain 200-fold (13Kim J.H. Krahn J.M. Tomchick D.R. Smith J.L. Zalkin H. Structure and function of the glutamine phosphoribosylpyrophosphate amidotransferase glutamine site and communication with the phosphoribosylpyrophosphate site..J. Biol. Chem. 1996; 271: 15549-15557Crossref PubMed Scopus (81) Google Scholar). TS and DHFR catalyze sequential reactions in the thymidylate pathway, which supplies 2-deoxythymidylate for DNA synthesis (Scheme FSI,D). Although TS and DHFR are distinct monofunctional enzymes in most species, the two activities are found on a single polypeptide chain in protozoa and some plants. DHFR-TS from Leishmania major is a homodimer, (α-β)2, with an N-terminal DHFR domain connected to the C-terminal TS domain. The 2.8-Å resolution x-ray structure of this bifunctional enzyme (10Knighton D.R. Kan C.C. Howland E. Janson C.A. Hostomska Z. Welsh K.M. Matthews D.A. Structure of and kinetic channeling in bifunctional dihydrofolate reductase-thymidylate synthase..Nat. Struct. Biol. 1994; 1: 186-194Crossref PubMed Scopus (203) Google Scholar) suggests a novel mechanism of “electrostatic channeling” across the surface of the protein. The negatively charged dihydrofolate intermediate is proposed to move along a positively charged electrostatic “highway” that links the TS active site to the DHFR site 40 Å away (10Knighton D.R. Kan C.C. Howland E. Janson C.A. Hostomska Z. Welsh K.M. Matthews D.A. Structure of and kinetic channeling in bifunctional dihydrofolate reductase-thymidylate synthase..Nat. Struct. Biol. 1994; 1: 186-194Crossref PubMed Scopus (203) Google Scholar, 11Stroud R.M. An electrostatic highway..Nat. Struct. Biol. 1994; 1: 131-134Crossref PubMed Scopus (47) Google Scholar) (Fig. 4). The proposed channeling mechanism is supported by the conservation of positively charged patches across the surface of monofunctional TS and DHFR from other sources, suggesting that the monofunctional enzymes may also channel substrates in vivo (11Stroud R.M. An electrostatic highway..Nat. Struct. Biol. 1994; 1: 131-134Crossref PubMed Scopus (47) Google Scholar). Electrostatic channeling in DHFR-TS is also supported by Brownian dynamics simulation and experimental kinetic studies (41Elcock A.H. Potter M.J. Matthews D.A. Knighton D.R. McCammon J.A. Electrostatic channeling in the bifunctional enzyme dihydrofolate reductase-thymidylate synthase..J. Mol. Biol. 1996; 262: 370-374Crossref PubMed Scopus (63) Google Scholar, 42Elcock A.H. Huber G.A. McCammon J.A. Electrostatic channeling of substrates between enzyme active sites: comparison of simulation and experiment..Biochemistry. 1997; 36: 16049-16058Crossref PubMed Scopus (64) Google Scholar, 43Meek T.D. Garvey E.P. Santi D.V. Purification and characterization of the bifunctional thymidylate synthetase-dihydrofolate reductase from methotrexate-resistant Leishmania tropica..Biochemistry. 1985; 24: 678-686Crossref PubMed Scopus (126) Google Scholar, 44Trujillo M. Donald R.G. Roos D.S. Greene P.J. Santi D.V. Heterologous expression and characterization of the bifunctional dihydrofolate reductase-thymidylate synthase enzyme of Toxoplasma gondii. Biochemistry. 1996; 35: 6366-6374Google Scholar). A recent transient kinetic analysis of DHFR-TS from L. major (15Liang P.H. Anderson K.S. Substrate channeling and domain-domain interactions in bifunctional thymidylate synthase-dihydrofolate reductase..Biochemistry. 1998; 37: 12195-12205Crossref PubMed Scopus (59) Google Scholar) demonstrates that the dihydrofolate intermediate is channeled efficiently as the result of two features of the reaction kinetics: the rate of dihydrofolate transfer between the two sites is very rapid (>1000 s−1) and each site is activated by ligand binding to the other site. Thus, reciprocal communication between the two sites leads to tight coupling of the reactions at the two sites. It is predicted that the highly negatively charged polyglutamylated molecules of dihydrofolate, which occur in cells (45Kisliuk R.L. Gaumont Y. Baugh C.M. Polyglutamyl derivatives of folate as substrates and inhibitors of thymidylate synthetase..J. Biol. Chem. 1974; 249: 4100-4103Abstract Full Text PDF PubMed Google Scholar), would be even more efficiently channeled than the monoglutamylated folates (10Knighton D.R. Kan C.C. Howland E. Janson C.A. Hostomska Z. Welsh K.M. Matthews D.A. Structure of and kinetic channeling in bifunctional dihydrofolate reductase-thymidylate synthase..Nat. Struct. Biol. 1994; 1: 186-194Crossref PubMed Scopus (203) Google Scholar). The crystal structure of DHFR-TS reveals that the negatively charged glutamate moieties of the folate analogs at the two sites lie in a groove along the electropositive highway between the two sites. The possibility that the bifunctional enzyme has evolved to enhance channeling is supported by the finding that the DHFR domain in the bifunctional enzyme has six extra positively charged residues located between the two sites, which may function in binding the polyglutamate tail. A comparison of the structural and kinetic features of the channeling enzymes described above indicates some common features. These enzymes are either multifunctional proteins or stable multienzyme complexes. Because allosteric signals coordinate the activities of each of these enzymes (12Anderson K.S. Miles E.W. Johnson K.A. Serine modulates substrate channeling in tryptophan synthase: a novel intersubunit triggering mechanism..J. Biol. Chem. 1991; 266: 8020-8033Abstract Full Text PDF PubMed Google Scholar, 13Kim J.H. Krahn J.M. Tomchick D.R. Smith J.L. Zalkin H. Structure and function of the glutamine phosphoribosylpyrophosphate amidotransferase glutamine site and communication with the phosphoribosylpyrophosphate site..J. Biol. Chem. 1996; 271: 15549-15557Crossref PubMed Scopus (81) Google Scholar, 14Pan P. Woehl E. Dunn M.F. Protein architecture, dynamics and allostery in tryptophan synthase channeling..Trends Biochem. Sci. 1997; 22: 22-27Abstract Full Text PDF PubMed Scopus (93) Google Scholar, 15Liang P.H. Anderson K.S. Substrate channeling and domain-domain interactions in bifunctional thymidylate synthase-dihydrofolate reductase..Biochemistry. 1998; 37: 12195-12205Crossref PubMed Scopus (59) Google Scholar, 16Miles B.W. Banzon J.A. Raushel F.M. Regulatory control of the amidotransferase domain of carbamoyl phosphate synthetase..Biochemistry. 1998; 37: 16773-16779Crossref PubMed Scopus (34) Google Scholar), allosteric communication may be a general and essential feature of channeling enzymes. These allosteric signals coordinate activities at two or more sites and promote efficient channeling that prevents the buildup of intermediates and their loss into solution. The four examples described above suggest that channeling may be a more general feature of biochemical processes involving enzyme complexes. The once isolated example of indole channeling to prevent the escape of the neutral indole from the cell is now supported by examples of channeling of reactive intermediates. A large number of enzymes have been suggested to form stable or transient complexes and to exhibit channeling or direct transfer of intermediates (2Ovadi J. Physiological significance of metabolite channeling..J. Theor. Biol. 1991; 152: 1-22Crossref PubMed Scopus (194) Google Scholar). For example, the finding of NH3 tunnels in two families of amidotransferases (GPATase and CPS) suggests that other amidotransferases in these families may have similar mechanisms of NH3 transfer (34Zalkin H. Smith J.L. Enzymes utilizing glutamine as an amide donor..Adv. Enzymol. Relat. Areas Mol. Biol. 1998; 72: 87-144PubMed Google Scholar). The products of these reactions may themselves be channeled to other enzymes. For example, there is kinetic and biochemical evidence for channeling between GPATase and glycinamide ribonucleotide synthetase (46Rudolph J. Stubbe J. Investigation of the mechanism of phosphoribosylamine transfer from glutamine phosphoribosylpyrophosphate amidotransferase to glycinamide ribonucleotide synthetase..Biochemistry. 1995; 34: 2241-2250Crossref PubMed Scopus (71) Google Scholar). This evidence is supported by docking experiments between the crystal structures of GPATase and glycinamide ribonucleotide synthetase (47Wang W. Kappock T.J. Stubbe J. Ealick S.E. X-ray crystal structure of glycinamide ribonucleotide synthetase from Escherichia coli. Biochemistry. 1998; 37: 15647-15662Google Scholar). Another example involves CPS, which catalyzes the first step in the pyrimidine biosynthetic pathway. In Saccharomyces cerevisiae, CPS is part of a larger polypeptide that catalyzes the first two steps in this pathway (CPS and aspartyl transcarbamoylase). In mammals, CPS is part of an even larger polypeptide, termed CAD, which catalyzes the first three steps in this pathway. The interaction of CPS and aspartyl transcarbamoylase has been proposed to promote effective coordination of the two activities and channeling of the labile carbamoyl phosphate intermediate in CAD (48Irvine H.S. Shaw S.M. Paton A. Carrey E.A. A reciprocal allosteric mechanism for efficient transfer of labile intermediates between active sites in CAD, the mammalian pyrimidine-biosynthetic multienzyme polypeptide..Eur. J. Biochem. 1997; 247: 1063-1073Crossref PubMed Scopus (30) Google Scholar) and in the enzyme from yeast (49Penverne B. Belkaid M. Herve G. In situ behavior of the pyrimidine pathway enzymes in Saccharomyces cerevisiae. 4. The channeling of carbamylphosphate to aspartate transcarbamylase and its partition in the pyrimidine and arginine pathways..Arch. Biochem. Biophys. 1994; 309: 85-93Crossref PubMed Scopus (26) Google Scholar).