Abstract
Crude lipase was extracted from defatted ground French bear seeds using 1×10 −3 M CaCl2 in combination with ultrasound disruption and further purified with ammonium sulfate at 60% of saturation. The additional use of ammonium sulfate doubled the crude lipase activity, while the combination of ultrasound and ammonium sulphate increased the activity by a factor of six. The effects of pH, calcium ion concentration and the addition of an emulsifying agent were also investigated. The optimum pH was determined to be near 7, and the addition of calcium ion beyond the concentration present in the extract had an inhibitory effect on the activity, whereas the addition of Tween-20 increased the activity of the enzyme by a factor of four. The specificity of French bean lipase, relative to pancreatic lipase, was studied using triacetin, olive oil and French bean lipid as substrates. The crude lipase fraction obtained from the French bean seed had a higher activity than pancreatic lipase and it also exhibited notable carboxulesterase activity.
Published Version
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